Unveiling the noncanonical activation mechanism of CDKs: insights from recent structural studies.

Front Mol Biosci

Department of Biochemistry and Molecular Biology, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX, United States.

Published: November 2023

AI Article Synopsis

  • Cyclin-dependent kinases (CDKs) are vital for key cellular processes, but CDK7 and CDK8 use unique mechanisms for activation compared to traditional cell cycle-related CDKs.
  • While binding to their cyclin partners is necessary for activity, full activation of CDK7 and CDK8 also requires an additional subunit for enhanced functionality.
  • Recent structural research has shed light on how this third subunit stabilizes key components of the kinases, paving the way for new drug discovery and treatments for diseases linked to these kinases.*

Article Abstract

The Cyclin-dependent kinases (CDKs) play crucial roles in a range of essential cellular processes. While the classical two-step activation mechanism is generally applicable to cell cycle-related CDKs, both CDK7 and CDK8, involved in transcriptional regulation, adopt distinct mechanisms for kinase activation. In both cases, binding to their respective cyclin partners results in only partial activity, while their full activation requires the presence of an additional subunit. Recent structural studies of these two noncanonical kinases have provided unprecedented insights into their activation mechanisms, enabling us to understand how the third subunit coordinates the T-loop stabilization and enhances kinase activity. In this review, we summarize the structure and function of CDK7 and CDK8 within their respective functional complexes, while also describing their noncanonical activation mechanisms. These insights open new avenues for targeted drug discovery and potential therapeutic interventions in various diseases related to CDK7 and CDK8.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10666765PMC
http://dx.doi.org/10.3389/fmolb.2023.1290631DOI Listing

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