AI Article Synopsis
- N. J. Bonde and colleagues conducted a study using unbiased transposon-sequencing to identify proteins needed for cell survival without the RecG helicase, revealing a case of synthetic lethality.
- The proteins identified are involved in pathways that prevent DNA damage and control the homology-directed repair process, focusing on resolving problematic intermediates that can occur between sister chromosomes.
- These findings highlight the importance of managing DNA damage to prevent dangerous accumulations that can interfere with proper chromosome segregation and lead to cell death.
Article Abstract
In this issue of the , N. J. Bonde, E. A. Wood, K. S. Myers, M. Place, J. L. Keck, and M. M. Cox (J Bacteriol 205:e00184-23, 2023, https//doi.org/10.1128/jb.00184-23) used an unbiased transposon-sequencing (Tn-seq) screen to identify proteins required for life when cells lose the RecG branched-DNA helicase (synthetic lethality). The proteins' identities indicate pathways that prevent endogenous DNA damage, pathways that prevent its homology-directed repair (HDR) "strand-exchange" intermediates between sister chromosomes, and pathways that resolve those intermediates. All avoid intermediate pile-up, which blocks chromosome segregation, causing "death-by-recombination." DNA damage is managed to regulate crucial but potentially lethal HDR.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10742612 | PMC |
| http://dx.doi.org/10.1128/jb.00272-23 | DOI Listing |
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