Buffalo milk processed by method of Kobata and Ginsburg for isolation of its oligosaccharide contents showed significant stimulation of anti-body, delayed type hypersensitivity response to sheep red blood cells in BALB/c mice. This oligosaccharide mixture also stimulated non-specific immune response in terms of MMI. In continuation to our previous studies of oligosaccharide contents of buffalo milk another novel pentasaccharide Ebaliose was isolated from immunostimulant oligosaccharide fraction of buffalo milk. Ebaliose was procured in its purest form, by combining the different chromatographic techniques like Gel filtration, Silica Gel column chromatography, and HPLC. The structure of novel oligosaccharide was determined by H,C, HSQC, TOCSY, COSY, HMBC, ESI-MS, chemical transformation, and chemical degradation. The stereoscopic structure of this novel pentasaccharide was established as under.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/14786419.2023.2284869 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Peptide Profile Changes in Buffalo Milk Cheese during Different Storage Periods and Characterization of Novel Bioactive Peptides through Peptidomics.
J Agric Food Chem
January 2025
College of Food Science & Technology, Yunnan Agricultural University institution, Kunming, Yunnan 650201, China.
This study aimed to investigate the changes in the bioactive peptides (BPs) of buffalo milk cheese (BMC) within 15 days of storage. A total of 3605 peptides were identified in the BMC, with 260 peptides remaining stable for 15 days. Among these, the peak intensities of all reported BPs (9 peptides) increased on the 15th day.
View Article and Find Full Text PDFThe Microbial Diversity and Traceability Analysis of Raw Milk from Buffalo Farms at Different Management Ranks in Guangxi Province.
Foods
December 2024
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China.
Farm management has a significant impact on microbial composition and may affect the quality of raw buffalo milk. This study involved a diversity analysis and traceability of the microbial communities in raw buffalo milk from three buffalo farms at different management ranks in Guangxi Province, China. The microbial composition of the raw buffalo milk and its environmental sources were investigated using 16S rRNA gene sequencing and bioinformatics analysis.
View Article and Find Full Text PDFSaccharomyces Cerevisiae Diet Supplementation Affects Nutrient Digestibility and Milk and Mozzarella Cheese Yield in Dairy Buffalo Cows During the Transition.
Animals (Basel)
December 2024
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, Italy.
The aim of the trial was to evaluate the influence of diet supplementation with Saccharomyces cerevisiae on nutrient digestibility and milk and mozzarella cheese yield in buffalo cows during the transition period. Twenty buffalo cows in the last month of pregnancy were equally divided into two groups (control, C, and treated, T) homogeneous for parity and milk yield at the previous lactation. The diet of group T was supplemented with 100 g/head/day of a product containing Saccharomyces cerevisiae (Sc 47- CNCM I-4407) for 4 weeks before and after calving.
View Article and Find Full Text PDFMethane Emission of Italian Mediterranean Buffaloes Measured Using a Laser Detector During a Lactation Cycle.
Animals (Basel)
December 2024
Consiglio per la Ricerca in Agricoltura e L'Analisi dell'Economia Agraria, Centro di Ricerca per la Zootecnia e L'acquacoltura, Monterotondo, 00015 Rome, Italy.
In Italy, the number of farmed dairy buffaloes rose up to approximately 436,000 heads in 2023 (+22% in the last 15 years), a fourfold increase compared to the 1980s, due to the growing market interest in mozzarella cheese. The increased demand for mozzarella cheese, in turn, requires higher production, which can result in increased methane emission from the sector. Therefore, it is necessary to establish mitigation and selection schemes for low-emission strategies.
View Article and Find Full Text PDFOccurrence and Molecular Characterization of Cryptosporidium spp. and Giardia duodenalis in Water Buffaloes (Bubalis bubalis) From Türkiye.
Acta Parasitol
January 2025
Department of Parasitology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Türkiye.
Article Synopsis
- Cryptosporidium and Giardia are common protozoan parasites affecting the digestive systems of various animals, leading to economic losses in animal breeding and posing public health risks.
- Recent studies identified numerous genotypes of these pathogens, prompting research into their prevalence in water buffaloes in Türkiye, where previous research was limited.
- The study found Cryptosporidium spp. in 3.92% and Giardia duodenalis in 0.98% of water buffalo stool samples, with C. ryanae being the most prevalent species, showing high genetic similarity among samples.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!