Background: Tracheotomy is a common technique; however, microbiological contamination of the surgical site can increase morbimortality. Up to 90% of patients present a positive culture of the airway. Among the most important related factors is the lack of tracheal cannula replacement and lower airway infections. It is convenient to identify microbiological contamination of surgical site in tracheal secretions samples and the specific microorganism associated.
Objective: To determine the factors related to microbiological contamination of surgical site.
Material And Methods: A prospective cohort study which included patients undergoing tracheotomy was carried out. Tracheal secretion was sampled by direct swabbing for culture during surgery and from the surgical site 5 days after.
Results: The initial report showed contamination of samples in 58.3%, and 5 days after in 80.6%, with an incidence of contamination of 22.3%. Initially the main agents identified were Pseudomonas aeruginosa in 13.9% of the cultures, Klebsiella pneumoniae in 11.1% and Enterobacter spp. in 11%. On day 5, the most common agents were Klebsiella pneumoniae in 25% of the cases, Acinetobacter baumannii in 11.1% and Pseudomonas aeruginosa in 11.1.
Conclusions: The frequency of microbiological contamination is high. The main agents were Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. No risk factors for the presence of post-tracheotomy contamination were identified.
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Am J Infect Control
January 2025
Nursing School, Universidade Estadual de Campinas (Unicamp), Faculdade de Enfermagem - Universidade Estadual de Campinas (Unicamp). Rua Tessália Vieira de Camargo, 126 - Cidade Universitária Zeferino Vaz. CEP 13083-887, Campinas, São Paulo, Brazil. Electronic address:
Background: The presence of microorganisms in laryngoscopes emphasizes the risk to patient safety during orotracheal intubations.
Methods: Cross-sectional study was carried out in university hospital in the inpatient, emergency, intensive care and surgical center sectors. Microorganisms were recovered from the blades using a filter membrane and from the handles using swab.
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