Analysis of virulence genes (PhlA, ShlA, FlhD) sequencing of Serratia marcescens including collection of two hundred twenty samples from sputum & wound infection of the period from April-June in 2021 of the patients in some hospitals in Baghdad - Iraq. These specimens were collected from central hospitals in Iraq. After laboratory diagnosis of these specimens by detecting morphological and biochemical tests on bacteria that were cultured on selective and enriched media, VITEK- 2 compact system. There are 40 bacterial isolates of Serratia marcescens from total samples (220) in percentage (18.18%). The genome of these bacteria was extracted to investigate target virulence genes that were amplified by specific forward and specific primers. The product size of virulence genes was Ph1A (207 bp), Sh1A (217 bp), and FlhD virulence gene (307 bp). The results exhibited that these isolates contained these genes at different levels. Sequencing of these genes was carried out and analyzed through BLAST in NCBI and Geneious version -9. The results explained the top identity of sequencing these virulence genes (PhlA, ShlA, FlhD) between local Iraqi bacteria. In addition, there are misidentify or dissimilarities in different levels between Iraqi S. marcescens and global strain recorded in NCBI. These results consider scientific evidence to find new variations of these virulence genes in Iraqi S. marcescens in comparison with the global strain. These new Iraqi bacterial variation sequencing registered in the global database in NCBI under accession numbers including (Ph1A virulence gene LC647828.1 & LC647829.1), (Sh1A virulence gene LC647830.1 & LC647831.1) & (F1hD virulence gene LC647826.1, LC647827.1). The results of analysis sequencing exhibited different percentages in genetic identity distance, which refer to these bacteria new variation in Pathogenicity Island. These results explained the ability of these bacteria to produce different levels of virulence factors that lead to an increase in pathogenicity and spreading of infection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.14715/cmb/2023.69.11.24 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A novel polysaccharide in the envelope of influences the septal secretion of preproteins with a YSIRK/GXXS motif.
J Bacteriol
January 2025
Department of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.
Unlabelled: Bacteria transport proteins across the plasma membrane to assemble their envelope, acquire nutrients, and establish appropriate interactions with their environment. The majority of these proteins are synthesized as precursors with a cleavable N-terminal signal sequence for recognition by the Sec machinery. In , a small subset of secreted precursors carries a YSIRK/GXXS motif.
View Article and Find Full Text PDFRibosome profiling reveals differences in global translational vs transcriptional gene expression changes during early biofilm formation.
Microbiol Spectr
January 2025
Department of Microbiology, Immunology & Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.
a major human fungal pathogen, can form biofilms on a variety of inert and biological surfaces. biofilms allow for immune evasion, are highly resistant to antifungal therapies, and represent a significant complication for a wide variety of immunocompromised patients in clinical settings. While transcriptional regulators and global transcriptional profiles of biofilm formation have been well-characterized, much less is known about translational regulation of this important virulence property.
View Article and Find Full Text PDFc-di-GMP phosphodiesterase ProE interacts with quorum sensing protein PqsE to promote pyocyanin production in .
mSphere
January 2025
School of Medicine, Southern University of Science and Technology, Shenzhen, China.
The universal bacterial second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) plays critical roles in regulating a variety of bacterial functions such as biofilm formation and virulence. The metabolism of c-di-GMP is inversely controlled by diguanylate cyclases (DGCs) and phosphodiesterases (PDEs). Recently, increasing studies suggested that the protein-protein interactions between DGCs/PDEs and their partners appear to be a common way to achieve specific regulation.
View Article and Find Full Text PDFGenetic diversity and virulence of group isolates from bloodstream infections.
Microbiol Spectr
January 2025
Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo, Japan.
catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of isolates from CRBSIs remain understudied. Here, using advanced genomic techniques, we investigated the genetic diversity, phylogenetic relationships, and virulence profiles of isolates from patients with bloodstream infections.
View Article and Find Full Text PDFPathogenicity assessment of genetic variants identified in patients with severe hypertriglyceridemia: novel cases of Familial Chylomicronemia Syndrome from the Dyslipidemia Registry of the Spanish Atherosclerosis Society.
Genet Med
January 2025
Lipids and Atherosclerosis Laboratory, Department of Medicine and Dermatology, Centro de Investigaciones Médico Sanitarias (CIMES), Instituto de Investigación Biomédica de Málaga y Plataforma en Nanomedicina (IBIMA -Plataforma Bionand), University of Málaga, Málaga, Spain; Lipid Unit. Internal Medicine Service. University Hospital Virgen de la Victoria, Málaga, Spain.
Purpose: Genetic testing is required to confirm a diagnosis of familial chylomicronemia syndrome (FCS). We assessed the pathogenicity of variants identified in the FCS canonical genes to diagnose FCS cases.
Methods: 245 patients with severe hypertriglyceridemia underwent next-generation sequencing.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!