The formation of radical anions (PDI 1˙) using HS as a sacrificial electron donor in 50% HEPES buffer-THF solution is reported. PDI 1˙ was confirmed by optical, - plot, CV, DPV, NOBF and EPR studies. PDI 1˙ has a half-life of 96 minutes in solution and 11 days in the solid state without any additive. The formation of PDI 1˙ was confirmed by AFM and SEM. PDI 1˙ can be used for the detection of 26.6 pM of HO supported by optical and CV data.
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http://dx.doi.org/10.1039/d3cc03690a | DOI Listing |
J Virol
May 2007
Virology Division, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
Stable trimeric forms of human immunodeficiency virus recombinant gp140 (rgp140) are important templates for determining the structure of the glycoprotein to assist in our understanding of HIV infection and host immune response. Such information will aid the design of therapeutic drugs and vaccines. Here, we report the production of a highly stable and trimeric rgp140 derived from a HIV type 1 (HIV-1) subtype D isolate that may be suitable for structural studies.
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