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sp. nov., an endophytic bacterium isolated from L. in wetland. | LitMetric

sp. nov., an endophytic bacterium isolated from L. in wetland.

Int J Syst Evol Microbiol

State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, PR China.

Published: November 2023

AI Article Synopsis

  • A new strain of bacteria, C31, was isolated from a wetland plant near Poyang Lake in China, characterized as Gram-stain-positive and aerobic with a yellowish, glossy appearance.
  • Phylogenetic analysis revealed that C31 has less than 97.0% similarity to known species in its genus, with low genomic identity and hybridization values, indicating it is a distinct species.
  • Optimal growth conditions for C31 are at 37°C, pH 7, and 0.5% NaCl, with specific fatty acids and polar lipids identified, leading to its classification as a novel species named sp. nov. with strain designation C31.

Article Abstract

A Gram-stain-positive, aerobic, rod-shaped, non-motile, yellowish and glossy strain, C31, was isolated from a wetland plant L. located south of Poyang Lake, Jiangxi Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C31 showed similarity values of lower than 97.0 % to other type species belonging to the genus . The genomic average nucleotide identity values between strain C31 and its reference type species ranged from 68.9-70.9 % and the digital DNA-DNA hybridization values were lower than 27.8 %. The genomic DNA G+C content of strain C31 was 41.9 mol%. The optimal growth temperature, pH and NaCl concentration were 37 °C, pH 7 and 0.5 %, respectively. The major cellular fatty acids (>5.0 %) of strain C31 were anteiso-C (73.7 %), anteiso-C (8.4 %) and iso-C (5.2 %). The polar lipids of strain C31 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified phospholipids. The respiratory quinone was MK-7. Based on these phylogenetic and phenotypic characterizations, strain C31 represents a novel species within the genus . Therefore, the proposed name for this newly identified species is sp. nov. and the type strain is C31 (=CCTCC AB 2022349=KCTC 43565).

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Source
http://dx.doi.org/10.1099/ijsem.0.006185DOI Listing

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