AI Article Synopsis
- Electron cryomicroscopy is a method for determining the structures of most biological molecules but faces challenges like access, preparation, and cost.
- A newly developed 100 keV instrument improves electron optics, detection, and processing, significantly reducing costs and time for structure determination to just hours.
- This instrument demonstrated its effectiveness by accurately imaging eleven specimens, ranging from 140 kDa to 2 MDa, using much less data than traditional methods, potentially broadening the scope of structural biology research.
Article Abstract
Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV-including advances in electron optics, detection, and processing-that makes structure determination fast and simple at a fraction of current costs. The instrument attains its theoretical performance limits, allowing atomic resolution imaging of gold test specimens and biological molecular structure determination in hours. We demonstrate its capabilities by determining the structures of eleven different specimens, ranging in size from 140 kDa to 2 MDa, using a fraction of the data normally required. CryoEM with a microscope designed specifically for high-efficiency, on-the-spot imaging of biological molecules will expand structural biology to a wide range of previously intractable problems.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710074 | PMC |
| http://dx.doi.org/10.1073/pnas.2312905120 | DOI Listing |
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