Single Cell Sequencing Analysis for Blood Tissue.

Single-cell sequencing is a technique based on sequencing genomic, transcriptomic and epigenomic analysis at the level of individual cells. Compared with traditional sequencing techniques, it can study intercellular heterogeneity and provide in-depth information on cell distribution, cell types and cell function of a specific population. This paper used blood tissue samples to analyze single-cell sequencing. Cells were downscaled by principal component analysis (PCA) and used Leiden clustering to discover cell heterogeneity in the same cluster and annotated after screening marker genes. Finally, 13 marker genes MTRNR2L8, NKG7, FLNA, IL7R, CCR7, CD8B, MKI67, LGALS3, TYROBP, C0251_TotalSeqC, CD79A, HLA-C, CCL4, and MCM5 in the experimental samples were found by cellular enrichment, which are strongly associated with white stuffy syndrome, skin damage, giant cell virus infection and other diseases are closely linked. In the medical field, the experimental results can be used to study the molecular characteristics and functional differences of different cell types under physiological and pathological conditions, thus providing insight into the pathogenesis and treatment of diseases such as leukoaraiosis syndrome, skin damage, and cytomegalovirus infection.