AI Article Synopsis
- ALKBH5 is highly expressed in gastric cancer (GC) and its silencing impairs cell proliferation, reduces metastatic potential, and induces cell death.
- The study identifies alterations in target genes, particularly CHAC1, due to ALKBH5's demethylation effects, which destabilize CHAC1 RNA and decrease its expression.
- CHAC1 affects intracellular ROS levels and plays a role in chemotherapy sensitivity, highlighting the important ALKBH5-CHAC1-ROS axis in the context of m6A methylation in gastric cancer.
Article Abstract
The m6a demethyltransferase ALKBH5 dynamically modulates gene expression and intracellular metabolic molecules by modifying RNA m6a in cancer cells. However, ALKBH5's function in gastric cancer (GC) has remained controversial. This study demonstrates that ALKBH5 is highly expressed in GC. Silencing ALKBH5 hampers proliferation, and metastatic potential, and induces cell death in GC cells. Through a comprehensive analysis of the transcriptome and m6A sequencing, alterations in certain ALKBH5 target genes, including CHAC1, were identified. ALKBH5's demethylation effect regulates CHAC1 RNA stability, leading to reduced CHAC1 expression. Moreover, CHAC1 modulates intracellular ROS levels, influencing the chemotherapy sensitivity of gastric cancer. In summary, our study unveils the pivotal role of the ALKBH5-CHAC1-ROS axis and highlights the significance of m6A methylation in gastric cancer.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676604 | PMC |
| http://dx.doi.org/10.1186/s12935-023-03129-9 | DOI Listing |
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