AI Article Synopsis
- The study investigates the protective effects of peimisine on mice with ulcerative colitis, focusing on its ability to mitigate inflammation and restore gut microbiota balance.
- Researchers used various methods, including staining techniques and molecular analysis, to evaluate peimisine's effects on disease activity, colon length, and cellular mechanisms.
- Findings indicate that peimisine reduces disease severity, prevents colon damage, modulates immune responses by impacting specific cytokine levels, and promotes beneficial gut bacteria while reducing harmful ones.
Article Abstract
Objectives: Inflammatory cytokine secretion and gut microbiota dysbiosis play crucial roles in ulcerative colitis. In this research, the protective effects of peimisine on colitis mice were investigated.
Methods: The protective effects were evaluated by the disease activity index, colonic length, hematoxylin-eosin, and AB/PAS Staining. The protective mechanisms were analyzed by ELISA, Western-blot, immunohistochemistry staining, immunofluorescence staining, and 16S rRNA gene analysis.
Key Findings: The results showed that peimisine treatment could reduce the disease activity index, prevent colonic shortening, and alleviate colon tissue damage. Peimisine treatment also decreased the levels of MCP-1, IL-1β, IL-6, IFN-γ, TNF-α and affected macrophage polarization and Th17/Treg cell balance by downregulating the expression of jak1/2, p-jak1/2, stat1/3, and p-stat1/3. Moreover, peimisine treatment significantly increased the abundances of beneficial microbes (e.g. Ruminococcaceae UCG-014 and Lachnospiraceae_NK4A136_group) and decreased the abundances of harmful microbes (e.g. Bacteroides and Escherichia).
Conclusions: Peimisine can ameliorate colitis by inhibiting Jak-Stat signaling pathway, reversing gut microbiota alterations, suppressing macrophage M1 polarization, maintaining the Th17/Treg cell balance, and reducing sustained inflammatory cytokines-related inflammatory injury.
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Source |
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| http://dx.doi.org/10.1093/jpp/rgad091 | DOI Listing |
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