C-Fixation Techniques.

This protocol describes the application of cyclotron-generated [C]CO fixation reactions for direct C-carboxylation reactions and [C]CO for C-carbonylations. Herein we describe one-pot methods wherein the radioactive gas is first trapped in a reaction mixture at room temperature and atmospheric pressure prior to the radiolabeling reactions. Such procedures are widely applicable to numerous small molecules to form C-labeled carboxylic acids, amides, esters, ketones, oxazolidinones, carbamates, and ureas. The steps for C-fixation techniques described herein are tailored for a commercial automated synthesis unit and are readily adapted for routine radiopharmaceutical production.