A Genome-Wide Identification and Expression Analysis of the Casparian Strip Membrane Domain Protein-like Gene Family in in Response to p-HBA-Induced Continuous Cropping Obstacles.

Casparian strip membrane domain protein-like () genes are key genes for the formation and regulation of the Casparian strip and play an important role in plant abiotic stress. However, little research has focused on the members, characteristics, and biological functions of the patchouli gene family. In this study, 156 genes were identified at the whole-genome level. Subcellular localization predicted that 75.6% of proteins reside on the cell membrane. A phylogenetic analysis categorized genes into five subclusters alongside genes. In a cis-acting element analysis, a total of 16 different cis-elements were identified, among which the photo-responsive element was the most common in the gene family. A transcriptome analysis showed that p-hydroxybenzoic acid, an allelopathic autotoxic substance, affected the expression pattern of , including a total of 27 upregulated genes and 30 down-regulated genes, suggesting that these may play an important role in the regulation of patchouli continuous cropping obstacles by affecting the formation and integrity of Casparian strip bands. These results provided a theoretical basis for exploring and verifying the function of the patchouli gene family and its role in continuous cropping obstacles.