Ink disease is considered one of the most significant causes contributing to the decline of chestnut orchards. The reduced yield of Mill can be attributed to two main species: and , with the first being the main pathogen responsible for ink disease in Portugal. is a highly aggressive and widely distributed plant pathogen, capable of infecting nearly 1000 host species. This oomycete causes substantial economic losses and is accountable for the decline of numerous plant species in Europe and worldwide. To date, no effective treatments are available to combat these pathogens. Given chestnut's economic and ecological significance, particularly in Portugal, it is crucial to investigate the molecular mechanisms underlying the interaction between species and host plants. This can be achieved through the study of the glucanase inhibitor protein (GIP) produced by during infection. The technique of RNA interference (RNAi) was employed to suppress the gene of . The resulting transformants, carrying the silenced gene, were used to infect , allowing for the assessment of the effects of gene silencing on the plant's phenotype. Additionally, bioinformatics tools predicted the secretion of GIP protein. The obtained results validate RNAi as a potential alternative tool for studying molecular factors and for controlling and managing .
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http://dx.doi.org/10.3390/plants12223821 | DOI Listing |
Commun Biol
January 2025
Institute of Phytopathology, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.
In vertebrates and plants, dsRNA plays crucial roles as PAMP and as a mediator of RNAi. How higher fungi respond to dsRNA is not known. We demonstrate that Magnaporthe oryzae (Mo), a globally significant crop pathogen, internalizes dsRNA across a broad size range of 21 to about 3000 bp.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Guizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Institute of Entomology, Guizhou University, Guiyang, Guizhou 550025, China. Electronic address:
RNA interference (RNAi) is a promising method for pest control; however, some studies have showed that the degradation of double-stranded RNA (dsRNA) by dsRNA-degrading nucleases (dsRNases) is one of the factors that may reduce RNAi efficiency in lepidopteran insects. In this study, we cloned two dsRNase genes named CmdsRNase5 and CmdsRNase6 from rice leaffolder Cnaphalocrocis medinalis, a notorious insect pest of rice. Open reading frames (ORFs) of CmdsRNase5 and CmdsRNase6 are 1317 and 1185 bp in length, encoding 438 and 394 amino acids, respectively.
View Article and Find Full Text PDFLancet Neurol
February 2025
Department of Neurosciences, and Leuven Brain Institute, University of Leuven, Leuven, Belgium; Laboratory of Neurobiology, Center for Brain & Disease Research, VIB, Leuven, Belgium. Electronic address:
Autosomal dominant mutations in the gene encoding the DNA and RNA binding protein FUS are a cause of amyotrophic lateral sclerosis (ALS), and about 0·3-0·9% of patients with ALS are FUS mutation carriers. FUS-mutation-associated ALS (FUS-ALS) is characterised by early onset and rapid progression, compared with other forms of ALS. However, different pathogenic mutations in FUS can result in markedly different age at symptom onset and rate of disease progression.
View Article and Find Full Text PDFBioorg Chem
January 2025
Department of In Vitro Carcinogenesis and Cellular Chemotherapy, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata 700026, India. Electronic address:
Histone deacetylases (HDACs) play a critical role in chromatin remodelling and modulating the activity of various histone proteins. Aberrant HDAC functions has been related to the progression of breast cancer (BC), making HDAC inhibitors (HDACi) promising small-molecule therapeutics for its treatment. Hydroxamic acid (HA) is a significant pharmacophore due to its strong metal-chelating ability, HDAC inhibition properties, MMP inhibition abilities, and more.
View Article and Find Full Text PDFPlant Physiol Biochem
January 2025
College of Horticulture, Henan Agricultural University, 218 Pingan Road, 450046, Zhengzhou, China; Henan Engineering and Technology Center for Peach Germplasm Innovation and Utilization, Zhengzhou, 450046, China; International Joint Laboratory of Henan Horticultural Crop Biology, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address:
The basic helix-loop-helix (bHLH) transcription factors (TFs) play important roles in various plant developmental and biological processes. However, the precise mechanisms by which bHLH TFs regulate fruit ripening warrant further investigation. Polyamine oxidase (PAO) is crucial for polyamine (PA) catabolism and plays crucial roles in fruit ripening.
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