Genome-Wide Characterization of Tomato Gene Family and Expression Analysis under Abiotic Stresses.

The fatty acid desaturase (FAD) gene family plays a crucial regulatory role in the resistance process of plant biomembranes. To understand the role of FADs in tomato growth and development, this study identified and analyzed the tomato gene family based on bioinformatics analysis methods. In this study, 26 were unevenly distributed on 10 chromosomes. Phylogenetic analysis showed that the gene family was divided into six branches, and the exon-intron composition and conserved motifs of clustered in the same branch were quite conservative. Several hormone and stress response elements in the promoter suggest that the expression of members is subject to complex regulation; the construction of a tomato FAD protein interaction network found that proteins have apparent synergistic effects with SPA and GPAT proteins. qRT-PCR verification results show that participates in the expression of tomato root, stem, and leaf tissues; is mainly highly expressed in leaves; plays a vital role in response to salt stress; and regulates all stages of fruit development under the action of exogenous hormones. In summary, this study provides a basis for a systematic understanding of the gene family. It provides a theoretical basis for in-depth research on the functional characteristics of tomato genes.