AI Article Synopsis
- - The study examined how blackcurrant (L.) leaf extracts possess antioxidant and anticancer properties by analyzing different fractions and their chemical compositions.
- - The extracts were divided into three fractions and tested for antiradical activity, total phenolic compounds, and anticancer effectiveness in two types of human cancer cells (colorectal and prostate).
- - Results indicated the F2 fraction had the highest phenolic content and antiradical activity, showing cytotoxic effects against colorectal cancer cells, suggesting blackcurrant leaves could serve as beneficial natural food additives.
Article Abstract
The aim of this study was to determine the relationship between antioxidant and anticancer properties of extracts from blackcurrant ( L.) leaves and their fractions and chemical contents. Dried ethanolic extract was divided into three fractions using solid phase extraction: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3). Both the extract and the fractions were analyzed in terms of antiradical activity (DPPH and ABTS), total phenolic compounds, and total flavonoids. The antitumor potential of the fractions was evaluated in vitro on human colorectal (HCT 116) and prostate (PC-3) cancer cells. Phenolics were identified using HPLC-QTOF-MS, and twelve compounds were quantified by HPLC-DAD. Finally, principal component analysis was carried out to assess the relationship between the tested factors. The results confirmed that blackcurrant leaves are a rich source of phenolics with high antioxidant activity and anticancer properties. It was demonstrated that the F2 fraction had the highest content of phenolics and the highest antiradical activity. Additionally, only this fraction showed cytotoxic activity against HCT 116 cells. It was confirmed that both the blackcurrant leaf extract and its fractions are a promising source of condensed active compounds and can be used as natural functional food additives.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673464 | PMC |
| http://dx.doi.org/10.3390/molecules28227459 | DOI Listing |
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