AI Article Synopsis
Article Abstract
Unexpected atypical isolates of occasionally challenge conventional microbiology and even the most advanced techniques for anthrax detection. For anticipating and gaining trust, 65 isolates of of diverse origin were sequenced and characterized. The BTyper3 tool was used for assignation to genomospecies (34), (29) and (2), as well as virulence factors and toxin profiling. None of them carried any capsule or anthrax-toxin genes. All harbored the non-hemolytic toxin and sphygomyelinase genes, whereas 41 (63%), 30 (46%), 11 (17%) and 6 (9%) isolates harbored , , and at least one insecticidal toxin gene, respectively. Matrix-assisted laser desorption ionization-time of flight mass spectrometry confirmed the production of cereulide ( genes). Phylogeny inferred from single-nucleotide polymorphisms positioned isolates relative to the lineage. One isolate (BC38B) was of particular interest as it appeared to be the closest neighbor described so far. It harbored a large plasmid similar to other previously described megaplasmids and at a lower extent to pXO1. Whereas bacterial collection is enriched, these high-quality public genetic data offer additional knowledge for better risk assessment using future NGS-based technologies of detection.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10673079 | PMC |
| http://dx.doi.org/10.3390/microorganisms11112721 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Brain-Derived Exosomal miR-9-5p Induces Ferroptosis in Traumatic Brain Injury-Induced Acute Lung Injury by Targeting Scd1.
CNS Neurosci Ther
December 2024
Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.
Aims: This study aimed to explore the role and underlying mechanisms of brain-derived exosomes in traumatic brain injury-induced acute lung injury (TBI-induced ALI), with a particular focus on the potential regulation of ferroptosis through miRNAs and Scd1.
Methods: To elucidate TBI-induced ALI, we used a TBI mouse model. Exosomes were isolated from the brains of these mice and characterized using TEM and NTA.
Conformational Space of 3-Chloropropionic Acid in Gas Phase Explored by Rotational Spectroscopy.
J Phys Chem A
December 2024
Departamento de Química Física y Química Inorgánica, Facultad de Ciencias─I.U. CINQUIMA, Paseo de Belén, 7, 47011 Valladolid, Spain.
The conformational space of 3-chloropropionic acid has been studied under the isolated conditions of a supersonic expansion using Stark-modulated free-jet absorption millimeter-wave and centimeter-wave chirped-pulse Fourier transform microwave spectroscopy techniques. The rotational spectra originating from the three most stable conformers including Cl and Cl isotopologues were observed in both experiments using helium expansion while a partial conformational relaxation involving skeletal rearrangements takes place in an argon expansion. The rotational parameters, geometries, and energy order were determined from the experiment, allowing a comparison with quantum chemical predictions.
View Article and Find Full Text PDFAssociation of Social Isolation and Plasma Metabolites With the Risk of Venous Thromboembolism.
Arterioscler Thromb Vasc Biol
December 2024
Division of Nephrology and State Key Laboratory of Organ Failure Research, National Clinical Research Center for Kidney Disease, Nanfang Hospital, Southern Medical University, Guangzhou, China. Guangdong Provincial Institute of Nephrology, Guangdong Provincial Key Laboratory of Renal Failure Research, Guangzhou, China.
Background: To explore the association of social isolation with venous thromboembolism (VTE) risk and the mediating role of metabolomics on this association.
Methods: Overall, 236 026 participants free of VTE and with complete metabolomics data at baseline from the UK Biobank were included. Social isolation was constructed based on the frequency of friend/family visits, weekly group activities, and living alone.
Dual-Stage Cross-Flow Filtration: Integrated Capture and Purification of Virus-Like Particles.
Biotechnol Bioeng
December 2024
Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Virus-like particles (VLPs) are a versatile technology for the targeted delivery of genetic material through packaging and potential surface modifications for directed delivery or immunological issues. Although VLP production is relatively simple as they can be recombinantly produced using microorganisms such as Escherichia coli, their current downstream processing often relies on individually developed purification strategies. Integrating size-selective separation techniques may allow standardized platform processing across VLP purification.
View Article and Find Full Text PDFScrotal Gangrene Secondary to Staphylococcus Aureus Infection: A Case Report.
Ann Ital Chir
December 2024
Department of Orthopedic, The First Affiliated Hospital of Zhejiang University, 310003 Hangzhou, Zhejiang, China.
Aim: To present a case of scrotal gangrene secondary to Staphylococcus aureus infection, highlighting the importance of early intervention and multidisciplinary care in the management of Fournier's gangrene.
Case Presentation: This case report details the clinical course of a 67-year-old male who presented with severe perineal pain and swelling, later diagnosed as Fournier's gangrene due to Staphylococcus aureus infection. The patient underwent emergency surgical debridement and received antibiotic therapy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!