Glycerol-3-phosphate acyltransferase GPAT9 catalyzes the first acylation of glycerol-3-phosphate (G3P), a committed step of glycerolipid synthesis in . The role of in remains to be elucidated. Here, we identified four orthologs of GPAT9 and found that BnaGPAT9 encoded by BnaC01T0014600WE is a predominant isoform and promotes seed oil accumulation and eukaryotic galactolipid synthesis in . is highly expressed in developing seeds and is localized in the endoplasmic reticulum (ER). Ectopic expression of BnaGPAT9 in and siliques of enhanced phosphatidic acid (PA) production. Overexpression of enhanced seed oil accumulation resulting from increased 18:2-fatty acid. Lipid profiling in developing seeds showed that overexpression of led to decreased phosphatidylcholine (PC) and a corresponding increase in phosphatidylethanolamine (PE), implying that BnaGPAT9 promotes PC flux to storage triacylglycerol (TAG). Furthermore, overexpression of also enhanced eukaryotic galactolipids including monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), with increased 36:6-MGDG and 36:6-DGDG, and decreased 34:6-MGDG in developing seeds. Collectively, these results suggest that ER-localized BnaGPAT9 promotes PA production, thereby enhancing seed oil accumulation and eukaryotic galactolipid biosynthesis in .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10671787 | PMC |
| http://dx.doi.org/10.3390/ijms242216111 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fresh Pineapple Agronomy in the Republic of Benin: Recent Trends in Calcium Carbide Use and Producer Perceptions.
Plant Environ Interact
February 2025
Genetics, Biotechnology and Seed Science Unit (GBioS), Laboratory of Crop Production, Physiology and Plant Breeding (PAGEV), Faculty of Agricultural Sciences University of Abomey-Calavi Cotonou Republic of Benin.
Pineapple ( (L.) Merrill) is among the main fruits produced in West Africa. This is also the case for the Republic of Benin, where pineapple fruit is regarded as an important crop for numerous producers in the Southern part of the country.
View Article and Find Full Text PDFDiversity and composition of ocular microbiota in contact lens wearers: Efficacy of liposomal ozonated oil.
Cont Lens Anterior Eye
January 2025
Department of Physics of Condensed Matter, Optics Area, University of Seville, Reina Mercedes S/N, 41012 Seville, Spain.
Purpose: To characterize the ocular surface microbiota in regular contact lens wearers with dry eyes and assess the effectiveness of reducing bacterial load using a liposomal ozonated oil solution.
Methods: This prospective, longitudinal, controlled study randomized subjects into two groups. Group A (45 subjects) received hydroxypropylmethylcellulose (HPMC, Artific®), while Group B (41 subjects) received ozonated sunflower seed oil with soybean phospholipids (OSSO, Ozonest®).
Physicochemical and functional properties of cinnamon essential oil emulsions stabilized by galactomannan-rich aqueous extract from Gleditsia sinensis seeds and soy protein isolate.
Int J Biol Macromol
January 2025
Technology Innovation Center of Natural Fragrances and Flavors, State Administration for Market Regulation, People's Republic of China.
Cinnamon essential oil has gained widespread attention in the food industry as a safe and effective preservative. However, its low water solubility and high volatility limit its application in food, making the use of natural emulsifiers for its emulsification an increasingly popular focus of research. This study focuses on the extraction of galactomannan-rich aqueous extracts from Gleditsia sinensis seeds using a low-energy, low-pollution microwave-assisted method.
View Article and Find Full Text PDFDiet supplementation with a mixture of essential oils: Effects on enteric methane emissions, apparent total-tract nutrient digestibility, nitrogen utilization, and lactational performance.
J Dairy Sci
January 2025
Agriculture and Agri-Food Canada, Quebec Research and Development Centre, Quebec, QC G1V 2J3 Canada.
This study examined the effects of supplementing dairy cows with a mixture of essential oils on enteric CH emissions, apparent total-tract nutrient digestibility, N utilization, and lactational performance (production, components and efficiency). Thirty-two multiparous lactating Holstein cows were used in a randomized complete block design. Cows averaged (mean ± SD) 95 ± 15.
View Article and Find Full Text PDFChemical profile changes in Peanut seeds infected with aspergillus flavus via widely targeted metabolomics.
Food Chem
January 2025
Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crop Genetic Improvement, South China Peanut Sub-Center of National Center of Oilseed Crops Improvement, Guangzhou, Guangdong Province 510640, China. Electronic address:
Peanut seeds are enriched with protein and fatty acids, making them susceptible to infection by Aspergillus flavus (A. flavus). The infected seeds are harmful to human health due to the aflatoxin contamination.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!