Developing magnetic functionalized dendritic fibrous mesoporous silica as advanced adsorbent for quaternary ammonium alkaloids.

A novel π-conjugated polymer-modified magnetic dendritic fibrous mesoporous silica adsorbent (MB@KCC-1@π-CP) is reported for the accurate determination of quaternary ammonium alkaloids (QAAs) in complex body fluid matrices. It is demonstrated that the magnetic dendritic fibrous mesoporous silica (MB@KCC-1) is an excellent carrier combining magnetism, high specific surface area, unique hierarchical pore structure, and fast mass transfer rate. The π-conjugated polymer (π-CP) can efficiently retain QAAs (berberine, coptisine, palmatine, jatrorrhizine) by multiple interactions. In addition, the adsorption kinetics and adsorption mechanism were also studied and discussed. Under optimized extraction conditions, MB@KCC-1@π-CP-based magnetic solid-phase extraction (MSPE) and high-performance liquid chromatography (HPLC) method affords a wide linear range (0.5-20000 ng mL), low limits of detection (0.2-2 ng mL), and satisfactory relative standard deviations (RSD) of inter-day (< 2.4%) and intra-day (< 3.1%) for QAAs. Trace QAAs in complex human blood plasma samples were successfully detected by the established method.