Alfalfa (Medicago sativa L.), a crucial and widely grown forage legume, faces yield and quality challenges due to salinity stress. The defender against apoptotic death (DAD) gene, recognized initially as an apoptosis suppressor in mammals, plays a pivotal role in catalyzing N-glycosylation, acting as a positive regulator for protein folding and endoplasmic reticulum (ER) export. Here, we found that the MsDAD2 gene was specially induced in the salt-tolerant alfalfa cultivar (DL) under salinity stress, but not in the salt-sensitive cultivar (SD). Overexpression of MsDAD2 enhanced the salinity resistance of transgenic alfalfa by promoting NAD(P)H-quinone oxidoreductase (NQO1) and cytochrome b6f complex subunit (Cyt b6/f) expression, thereby mitigating reactive oxygen species (ROS) production. ChIP-qPCR analysis suggested that the differential expression of MsDAD2 in DL and SD under salinity stress may be linked to dynamic histone modifications in its promoter. Therefore, our findings elucidate a novel regulatory mechanism of MsDAD2 in alfalfa's response to salinity stress, underscoring its significance as a target for alfalfa breeding to enhance salt tolerance.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2023.149252 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microbial hauberks: composition and function of surface layer proteins in gammaproteobacterial methanotrophs.
Appl Environ Microbiol
December 2024
Biology Department, San Diego State University, San Diego, California, USA.
Unlabelled: Many species of proteobacterial methane-consuming bacteria (methanotrophs) form a hauberk-like envelope represented by a surface (S-) layer protein (SLP) matrix. While several proteins were predicted to be associated with the cell surface, the composition and function of the hauberk matrix remained elusive. Here, we report the identification of the genes encoding the hauberk-forming proteins in two gamma-proteobacterial (Type I) methanotrophs, 5GB1 (EQU24_15540) and 20Z (MEALZ_0971 and MEALZ_0972).
View Article and Find Full Text PDFMicrobial Biotic Associations Dominated Adaptability Differences of Dioecious Poplar Under Salt Stress.
Plant Cell Environ
January 2025
Key Laboratory of the State Forestry and Grassland Administration for the Cultivation of Forests in the Lower Reaches of the Yellow River, College of Forestry, Shandong Agricultural University, Tai'an, China.
How different stress responses by male and female plants are influenced by interactions with rhizosphere microbes remains unclear. In this study, we employed poplar as a dioecious model plant and quantified biotic associations between microorganisms to explore the relationship between microbial associations and plant adaptation. We propose a health index (HI) to comprehensively characterize the physiological characteristics and adaptive capacity of plants under stress.
View Article and Find Full Text PDFIsolation and characterization of thermotolerant hydrocarbon degrading bacteria which sustained the activity at extreme salinity and high osmotic conditions.
Mol Biol Res Commun
January 2025
Department of Soil Science, College of Agriculture, Razi University, Kermanshah, Iran.
The bioremediation method is considered an economical and environmentally friendly strategy for the remediation of oil-contaminated soils. However, some oil field areas have extreme environmental conditions that make it difficult to establish microbes for bioreme-diation. In this study, bacteria were isolated from oil-contaminated soils of the Dehloran oil fields, which have very harsh soil and weather conditions.
View Article and Find Full Text PDFD1-104/3 and C31-106/3 differentially modulate the antioxidative response of duckweed ( L.) to salt stress.
Front Microbiol
December 2024
Department of Plant Physiology, Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.
Introduction: The common duckweed () is a model organism for investigation of plant physiology, especially stress-related responses. Its two physiological characteristics are of special interest: (1) salt-stressed duckweeds may accumulate starch, a precursor for biofuel; (2) duckweeds are associated with various beneficial (plant-growth promoting, PGP) bacterial strains. In this paper, we analyzed the role of two bacterial strains: D1-104/3 and C31-106/3 in regulation of duckweed's growth and antioxidative responses to salt (10 and 100 mM NaCl) and hypothesized that they alleviate salt-induced oxidative stress.
View Article and Find Full Text PDFThiourea induces antioxidant mechanisms of salt tolerance in flax plants.
Physiol Mol Biol Plants
December 2024
Science and Technology Department, University College in Nairiyah, University of Hafr Al Batin (UHB), 31991 Nairiyah, Saudi Arabia.
Salinity is one of the abiotic stress factors that affect plant physiology and cause various plant disorders. Thiourea, which consists of amino, thiol, and imino groups, is an antioxidant and growth regulator. The objective was to determine the antioxidant role of thiourea (0, 3, 6 mM) in attenuating the effects of salinity (0 mM, 50 mM, 100 mM NaCl) on growth, yield, and some biochemical compositions of flax ( L.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!