This article aims to review research updates and progress on the nutritional significance of the amides I and II, the alpha-helix and beta-sheet ratios, the microbial protein synthesis, and the steam pressure toasting condition in food and feed with globar and synchrotron molecular microspectroscopic techniques plus chemometrics (both univariate and multivariate techniques). The review focused on (I) impact of the amides I and II, and the alpha-helix and beta-sheet-structure ratios in food and feeds; (II) Current research progress and update in synchrotron technique and application in feed and food molecular structure studies that are associated with nutrition delivery; (III) Impact of thermal processing- steam pressure toasting condition on feed and food; (IV). Impact of the microbial protein synthesis and methodology on feed and food; and (V). Impact on performance and production of ruminants with Faba beans.
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http://dx.doi.org/10.1080/10408398.2023.2274442 | DOI Listing |
Electrophoresis
January 2025
Department of Medical Chemistry and Clinical Biochemistry, Second Faculty of Medicine, Charles University in Prague and Motol University Hospital, Prague, Czech Republic.
Hemoglobinopathies, hereditary disorders affecting the structure or production of hemoglobin, were detected by routine HbA measurements by capillary electrophoresis (CE) at the University Hospital Motol, Prague. The potential of ultraviolet-visible (UV-Vis) and Fourier-transform infrared (FTIR) spectroscopy for the detection and characterization of hemoglobinopathies was investigated. FTIR spectra were recorded with a very high resolution (0.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Zhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China. Electronic address:
Curcumin (Cur) has many potential applications in the food industry. However, it suffers from poor water solubility, low plasma and tissue uptake and susceptibility to oxidation. In this study, β-lactoglobulin (β-LG)-hyaluronic acid (HA) binary covalent complexes were prepared by ultrasound-assisted Maillard reaction and used as the carrier to deliver Cur.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Department of Food Engineering and Technology, Institute of Chemical Technology, Matunga, Mumbai, India. Electronic address:
α-amylase is responsible for shortening the shelf life of food by degrading starch and glycogen into maltose, disaccharides, and oligosaccharides. This study focuses on the effect of atmospheric cold plasma on the activity, stability, enzyme kinetics, and structural change for its application in α-amylase modifications. A reduction in the residual activity from 96.
View Article and Find Full Text PDFLuminescence
December 2024
Biophysical and Protein Chemistry lab, Department of Chemistry, NIT Rourkela, Rourkela, India.
Crowding environment has a significant impact on the folding and stability of protein in biological systems. In this work, we have used four different sizes of a molecular crowder, polyethylene glycol (PEG), to analyze the unfolding and refolding kinetics of an iLBP protein, CRABP I, using urea as chemical denaturant. In general, the stability of the native state of the protein is boosted by the presence of crowding agents in the solution.
View Article and Find Full Text PDFFood Res Int
December 2024
Drug Delivery and Nanomedicine Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER)-Raebareli, Lucknow, India. Electronic address:
In the current research work, zein-based polymeric nanocarriers were developed for the delivery of piperine, where the effect of non-ionic surfactants (Tween 80 and Pluronic F-127) on the stability and in vitro drug release characteristics were studied. Physiochemical characterization studies showed that the particle size of blank and drug-loaded surfactant stabilized zein nanoparticles were <200 nm and particles were spherical in shape. FTIR studies confirmed the successful incorporation of piperine within the zein nanoparticle without any alteration in primary structure of zein.
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