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Examining the effect of direct-from-blood bacterial testing on antibiotic administration and clinical outcomes: a protocol and statistical analysis plan for a pragmatic randomised trial.
BMJ Open
January 2025
Department of Medicine, Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
Introduction: Patients with suspected bacterial infection frequently receive empiric, broad-spectrum antibiotics prior to pathogen identification due to the time required for bacteria to grow in culture. Direct-from-blood diagnostics identifying the presence or absence of bacteria and/or resistance genes from whole blood samples within hours of collection could enable earlier antibiotic optimisation for patients suspected to have bacterial infections. However, few randomised trials have evaluated the effect of using direct-from-blood bacterial testing on antibiotic administration and clinical outcomes.
View Article and Find Full Text PDFSuccinic acid synthesis regulated by succinyl-coenzyme A ligase (SUCLA) plays an important role in root response to alkaline salt stress in Leymus chinensis.
Plant Physiol Biochem
January 2025
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China. Electronic address:
Alkaline salts have more severe adverse effects on plant growth and development than neutral salts do. However, the adaptive mechanisms of plants to alkaline salt stress remain poorly understood, especially at the molecular level. The Songnen Plain in northeast China is composed of typical 'soda' saline-alkali soil, with NaHCO and NaCO as the predominant alkaline salts (pH ≥ 9.
View Article and Find Full Text PDFInvestigations into the growth and formation of biofilm by at temperatures encountered during infection.
Biofilm
June 2025
Infectious Bacterial Diseases Research Unit, USDA Agriculture Research Service, National Animal Disease Center, Ames, IA, USA.
The genus comprises unique atypical spirochete bacteria that includes the etiological agent of leptospirosis, a globally important zoonosis. Biofilms are microecosystems composed of microorganisms embedded in a self-produced matrix that offers protection against hostile factors. Leptospires form biofilms in rice fields and unsanitary urban areas, and while colonizing rodent kidneys.
View Article and Find Full Text PDFAs the number and variety of assembled genomes continues to grow, the number of annotated genomes is falling behind, particularly for eukaryotes. DNA-based mapping tools help to address this challenge, but they are only able to transfer annotation between closely-related species. Here we introduce LiftOn, a homology-based software tool that integrates DNA and protein alignments to enhance the accuracy of genome-scale annotation and to allow mapping between relatively distant species.
View Article and Find Full Text PDFUnderstanding and Exploring the Food Preferences of Filipino School-Aged Children Through Free Drawing as a Projective Technique.
Nutrients
November 2024
MASLD UNIT, Fondazione Italiana Fegato, 34149 Trieste, Italy.
Background And Objectives: Numerous traditional and innovative approaches have been employed to understand and evaluate children's food preferences and food and nutrition knowledge, recognizing their essential role in shaping good nutrition. Drawing as a projective technique allows children to express their unconscious thoughts and preferences through visual representation, distinguishing it from other methods by providing an insight into their inner feelings and perceptions that may not be easily articulated through verbal techniques. The main goals of the study are to use drawing as a projective technique to gain insights into children's food preferences, and to examine the children's current nutrition knowledge and dietary perceptions.
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