Numerous species of plant viruses are naturally transmitted by insect vectors, mainly homopterans like aphids and whiteflies. Depending on the vector specificity and the mode of transmission, different durations of the periods for acquisition, retention, and inoculation are required for a successful transmission. Therefore, the experimental setup to perform controlled transmission experiments under laboratory conditions involves handling the vector organisms and managing the times for the different steps of the process to optimize and standardize the results. This chapter describes some basic procedures that can be applied to vector-mediated transmission experiments with selected viruses using aphids or whiteflies and different host plants.
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