AI Article Synopsis
- The study presents a method for efficiently synthesizing both regular and chemically altered trinucleotide triphosphates (dNTPs).
- This synthesis requires the use of phosphorothioate units to ensure stability for enzymatic DNA synthesis.
- The ability to utilize three-letter codons instead of single-letter nucleotides is advantageous for creating xenonucleic acids (XNAs) and selecting modified nucleic acids with specific functions.
Article Abstract
Herein, we report the high-yielding solid-phase synthesis of unmodified and chemically modified trinucleotide triphosphates (dNTPs). These synthetic codons can be used for enzymatic DNA synthesis provided their scaffold is stabilized with phosphorothioate units. Enzymatic synthesis with three rather than one letter nucleotides will be useful to produce xenonucleic acids (XNAs) and for selection of modified functional nucleic acids.
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| http://dx.doi.org/10.1039/d3cc04933g | DOI Listing |
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