gen. nov., sp. nov., isolated from marine sediment, and genomic comparison with multiple genera.

A Gram-stain-negative, rod-shaped, non-gliding, non-flagellated, yellow, facultatively aerobic bacterial strain, designated as W260, was isolated from marine sediment of Xiaoshi Island, Weihai, PR China. The cells of W260 were 0.3-0.5 µm wide and 1.5-2.0 µm long. Strain W260 grows optimally at a temperature of 33 °C (range, 15-37 °C), pH 8 (range, pH 6.5-9.5) and witha NaCl concentration of 3.0 % (w/v; range, 1-8 %). It has the highest sequence similarity to DSM 21152 (91.7 %), followed by MCCC 1K00261 (91.4 %) and DSM 17116 (90.7 %). The similarity between strain W260 and the species DSM 16326 was 89.4 %. Genome sequencing revealed a genome size of 3 430 000 bp and a DNA G+C content of 64.5 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain W260 and MCCC 1K00261 were 69.6 and 16.1-20.6 %, respectively. The predominant quinone was ubiquitin-8, and the major fatty acids were iso-C and iso-C. The polar lipids consisted of phosphatidylethanolamine, phospholipid, phosphatidylglycerol, diphosphatidylglycerol and four unidentified lipids. Based on phenotypic, phylogenetic and chemotaxonomic information, it was determined that strain W260 represents a novel genus and species and it was given the name sp. nov. The type strain is W260 (=MCCC 1H00471=KCTC 92639).