AI Article Synopsis
- - Organoids are tiny structures that act like real organs, and they're helpful for studying how our body reacts to germs and medicines in a way that’s more like what happens inside a living thing.
- - Scientists are especially interested in using these organoids to learn more about a fungus called Cryptococcus neoformans, which can make sick people even sicker, especially those with weak immune systems.
- - The research focuses on using special lung organoids made from stem cells to see how this fungus sticks to, invades, and grows in our lungs, as well as how our lungs' defenses respond to it.
Article Abstract
Organoids can meet the needs between the use of cell culture and in vivo work, bringing together aspects of multicellular tissues, providing a more similar in vitro system for the study of various components, including host-interactions with pathogens and drug response. Organoids are structures that resemble organs in vivo, originating from pluripotent stem cells (PSCs) or adult stem cells (ASCs). There is great interest in deepening the understanding of the use of this technology to produce information about fungal infections and their treatments. This work aims the use 2D human lung organoid derived from human embryonic stem cells (hESCs), to investigate Cryptococcus neoformans-host interactions. C. neoformans is an opportunistic fungus acquired by inhalation that causes systemic mycosis mainly in immunocompromised individuals. Our work highlights the suitability of human minilungs for the study of C. neoformans infection (adhesion, invasion and replication), the interaction with the surfactant and induction of the host's alveolar pro-inflammatory response.
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| http://dx.doi.org/10.1016/j.micinf.2023.105260 | DOI Listing |
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