Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland. Electronic address:
Published: March 2024
AI Article Synopsis
Article Abstract
Fragment of antigen-binding region (Fab) of antibodies are important biomolecules, with a broad spectrum of functionality in the biomedical field. While full length antibodies are usually produced in mammalian cells, the smaller size, lack of N-glycosylation and less complex structure of Fabs make production in microbial cell factories feasible. Since Fabs contain disulfide bonds, such production is often done in the periplasm, but there the formation of the inter-molecular disulfide bond between light and heavy chains can be problematic. Here we studied the use of the CyDisCo system (cytoplasmic disulfide bond formation in E. coli) to express two Fabs (Herceptin and Maa48) in the cytoplasm of E. coli in fed-batch fermentation using a generic chemically defined media. We were able to solubly express both Fabs with purified yields of 565 mg/L (Maa48) and 660 mg/L (Herceptin) from low density fermentation. Both proteins exhibited CD spectra consistent with natively folded protein and both were biologically active. To our knowledge this is the first demonstration of high-level production of biological active Fabs in the cytoplasm of E. coli in industrially relevant fermentation conditions.
protein design is delivering new peptide and protein structures at a rapid pace. Many of these synthetic polypeptides form well-defined and hyperthermal-stable structures. Generally, however, less is known about the dynamic properties of the designed structures.
The final disposal of corpses is one of the activities that generates the greatest pollution. Chemicals, embalming fluids, greenhouse gas emissions, and other factors trigger contamination of soils and water sources in towns near cemeteries. This study aimed to validate empirical environmental equations to determine the suitability of territories for cemetery location in several cantons of Central Ecuador, address study variables measured in the field, and update the bibliography as a rapid decision-making tool for decentralized governments.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, 30 Puzhu Road, Nanjing, 211816, P. R. China.
Crystalline membranes, represented by the metal-organic framework (MOF) with well-defined angstrom-sized apertures, have shown great potential for molecular separation. Nevertheless, it remains a challenge to separate small molecules with very similar molecular size differences due to angstrom-scale defects during membrane formation. Herein, a stepwise assembling strategy is reported for constructing MOF membranes with intrinsic angstrom-sized lattice aperture lattice to separate organic azeotropic mixtures separation.
Background: Sodium-glucose cotransporter-2 inhibitors (SGLT2i) have been proven to prevent decline in kidney function and failure. Whether SGLT2i affect the risk of contrast-associated acute kidney injury (CA-AKI) remains uncertain.
Methods: Use of SGLT2i was assessed in consecutive diabetics undergoing coronary angiography (CA) or percutaneous coronary intervention (PCI) from January 2020 to May 2023 at a tertiary hospital in Chongqing, China.
The applications of polymeric membranes have grown rapidly compared to traditional separation technologies due to their energy efficiency and smaller footprint. However, their potential is not fully realized due, in part, to their heterogeneity, which results in a "permeability-selectivity" trade-off for most membrane applications. Inspired by the intricate architecture and excellent homogeneity of biological membranes, bioinspired and biomimetic membranes (BBMs) aim to emulate biological membranes for practical applications.
