Evoked brain oscillations in the gamma range have been shown to assist in stroke recovery. However, the causal relationship between evoked oscillations and neuroprotection is not well understood. We have used optogenetic stimulation to investigate how evoked gamma oscillations modulate cortical dynamics in the acute phase after stroke. Our results reveal that stimulation at 40 Hz drives activity in interneurons at the stimulation frequency and phase-locked activity in principal neurons at a lower frequency, leading to increased cross-frequency coupling. In addition, 40-Hz stimulation after stroke enhances interregional communication. These effects are observed up to 24 h after stimulation. Our stimulation protocol also rescues functional synaptic plasticity 24 h after stroke and leads to an upregulation of plasticity genes and a downregulation of cell death genes. Together these results suggest that restoration of cortical dynamics may confer neuroprotection after stroke.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.celrep.2023.113475 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Neural recording chambers for long-term access to brain tissue.
J Neurosci
December 2024
Department of Neuroscience, The University of Texas at Austin, Austin, USA, 78712.
We describe a chamber system to perform imaging, electrophysiology and optogenetic stimulation in awake and anaesthetized marmosets. We developed this low-profile chamber design to be able to access the underlying tissue when needed, or to leave it sealed for long periods. Such accessibility is useful to maintain chamber clarity as well as perform viral or drug injections at different time points.
View Article and Find Full Text PDFModular Light-Emitting Diode Shelving Systems for Scalable Optogenetics.
Methods Mol Biol
December 2024
Flinders Health and Medical Research Institute (FHMRI), College of Medicine and Public Health, Flinders University, Bedford Park, SA, Australia.
Optogenetic experiments rely on the controlled delivery of light to diverse biological systems. Impressive devices have been recently developed to stimulate cells and small animals with multiple wavelengths and intensities. However, existing hardware solutions are often limited to a single sample holder, and their design and cost can further limit scalability.
View Article and Find Full Text PDFAutomated Plate Reader-Based Assays of Light-Activated GPCRs.
Methods Mol Biol
December 2024
Australian Regenerative Medicine Institute (ARMI), Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, VIC, Australia.
In the emerging field of optogenetics, light-sensitive G-protein coupled receptors (GPCRs) allow for the temporally precise control of canonical cell signaling pathways. Expressing, stimulating, and measuring the activity of light-sensitive GPCRs (e.g.
View Article and Find Full Text PDFLight-Inducible Deformation of Mitochondria in Live Cells.
Methods Mol Biol
December 2024
Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, China.
Mitochondria are dynamic organelles with constantly changing morphologies. Despite recent reports indicating that mechanical cues modulate mitochondrial morphologies and functions, there is a lack of methods that can exclusively and precisely exert mechanical forces to and deform mitochondria in live cells. Therefore, how mitochondria sense and respond to mechanical forces remains largely elusive.
View Article and Find Full Text PDFOptogenetic Clustering and Stimulation of the T Cell Receptor in Nongenetically Modified Human T Cells.
Methods Mol Biol
December 2024
INM-Leibniz Institute for New Materials, Saarbrücken, Germany.
Methods for the precise temporal control of cell surface receptor activation are indispensable for the investigation of signaling processes in mammalian cells. Optogenetics enables such precise control, but its application in primary cells is limited by the imperative for genetic manipulation of target cells. We here describe a method that overcomes this obstacle and enables the precise activation of the T cell receptor in nongenetically engineered human T cells by light.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!