Transcriptional Response of to Nanostructured Surfaces Provides Insight into Cellular Rupture and Antifungal Drug Sensitization.

The rise in resistance levels against antifungal drugs has necessitated the development of strategies to combat fungal infections. Nanoscale antimicrobial surfaces, found on the cuticles of insects, have recently emerged as intriguing alternative antifungal strategies that function passively via contact and induced cell rupture. Nanostructured surfaces (NSS) offer a potentially transformative antimicrobial approach to reducing microbial biofilm formation. We examined the transcriptional response of , an opportunistic pathogen that is also a commensal dimorphic fungus, to the NSS found in the wings of spp. cicada and found characteristic changes in the expression of genes associated with metabolism, biofilm formation, ergosterol biosynthesis, and DNA damage response after 2 h of exposure to the NSS. Further validation revealed that these transcriptional changes, particularly in the ergosterol biosynthesis pathway, sensitize to major classes of antifungal drugs. These findings provide insights into NSS as antimicrobial surfaces and as a means of controlling biofilm formation.