AI Article Synopsis
Article Abstract
Objective: To investigate the protective effect of Illicium verum extract on the vascularization of osteoporotic fracture in rats, and to elucidate its potential mechanism.
Methods: The osteoporotic fracture model was established in ovariectomized rats. Rats were infused with 0.05 ml/kg extract in the stomach every morning. Eighteen rats are then divided into control group, model group, and Illicium verum extract group with 6 rats in each group. To observe the therapeutic effect of Illicium verum extract on osteoporotic rats. Femoral bone mineral density and elastic segment end-point load were evaluated by dual-energy x-ray absorptiometry and three-point bending test. Hematoxylin-eosin staining was used to measure the number and area of callus blood vessels. The serum levels of VEGF and NO were detected by ELISA. Moreover, the expressions of NOX2, NOX4, NRF2, p-PI3K, CyclinD1, VEGF, HIF1α, and eNOS in HUVEC were detected by Western blot. CCK8 and wound healing assay were used to detect the proliferation and migration of HUVEC. Then, the ability of HUVEC to form blood vessels was detected by tube formation assay.
Results: Firstly, control group showed the normal pathomorphology and density of femoral bone, and model group showed significantly decreased bone density and consistent with bone microstructure degeneration, destruction, thinning, and fracture of bone trabecular structure vs control group, and illicium verum extract significantly increased femoral density and maximum load, increased the number and area of callus blood vessels and increased VEGF and NO levels in serum vs model group. Then, Illicium verum extract promoted the expression of NRF2, p-PI3K, CyclinD1, VEGF, HIF1α, and eNOS protein in HUVEC, inhibited the expression of NOX2 and NOX4, and enhanced the cell proliferation, migration, and angiogenesis. However, the effect was reversed by the overexpression of NRF2 and the treatment with LY294002.
Conclusion: Illicium verum extract protects the vascularization of the osteoporotic fracture model in rats.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
(Illiciaceae), an ecologically significant endemic plant, predominantly grows in Guangxi, China, which is the primary region for its cultivation. This area accounts for more than 80% of the total cultivation and yield in China. Despite its importance, comprehensive studies on the chloroplast (cp) genome of are limited.
View Article and Find Full Text PDFMolecular cloning and biochemical characterization of indole-3-acetic acid methyltransferase from Japanese star anise ().
Plant Biotechnol (Tokyo)
March 2024
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan.
SABATH proteins methylate the carboxyl groups or nitrogen atoms of small plant molecules and play important roles in many developmental processes and plant defense responses. Previous studies have shown that indole-3-acetic acid (IAA) carboxyl methyltransferase (IAMT), a member of the SABATH methyltransferase family, converts IAA into its methyl ester (Me-IAA). We used RNA-seq analysis to identify a putative gene, , in the ancient angiosperm .
View Article and Find Full Text PDFUltra-High-Performance Liquid Chromatography-High-Definition Mass Spectrometry-Based Metabolomics to Reveal the Potential Anti-Arthritic Effects of in Cultured Fibroblast-like Synoviocytes Derived from Rheumatoid Arthritis.
Metabolites
September 2024
National Engineering Research Center of Southwest Endangered Medicinal Resources Development, Guangxi Botanical Garden of Medicinal Plants, 189 Changgang Road, Nanning 530023, China.
Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. The fruits of , which is a medicinal and edible resource, have been shown to have anti-inflammatory properties.
Methods: In this study, we investigated the effects of extracts (IVEs) on human RA fibroblasts-like synoviocytes (RA-FLS) by using a sensitive and selective ultra-high-performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) method.
Chemical constituents from the stem bark of Illicium burmanicum and their anti-inflammatory activity.
Phytochemistry
January 2025
Department of Pharmacy, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China. Electronic address:
Thirty-six compounds were isolated from extract of the stem bark of Illicium burmanicum, including twelve previously undescribed prenylated C6-C3 compounds and a norsesquiterpene lactone: illicidione D (1), illicidione E (2), illicidione F (3), illicidione G (4), (2R,4S,11R)-12-O-ethylillifunone C (5), (2R,4S,11R)-illifunone C-12-O-β-d-glucopyranoside (6), (2R,4S,11R)-2-hydroxyillifunone C (7), 4-epi-2,3-dehydroillifunone C (8), illiburmanone A (9), illiburmanone B (10), illiburmanlactone A (11), (2S,4S,5S,11R)-2,3-dihydroillicione E (12), and illiburmanolside A (13). Their structures were determined based on extensive spectroscopic data analyses, including MS, NMR, and ECD spectra. The anti-inflammatory activity of the isolated compounds (1-36) was evaluated, and compounds 7, 12, 14, and 18 exhibited inhibitory effects in RAW 264.
View Article and Find Full Text PDFChloroplast mini-barcodes combined with high resolution melting analysis to identify herbal medicine Difengpi ().
Heliyon
October 2024
Guangxi Key Laboratory of Drug Discovery and Optimization, School of Pharmacy, Guilin Medical University, Guilin, 541004, China.
Article Synopsis
- Difengpi, a medicinal herb listed in the Chinese Pharmacopoeia, faces issues due to declining natural resources and the risk of being replaced by harmful substitutes, prompting the need for effective authentication methods.
- The study developed chloroplast mini-barcoding and high resolution melting (HRM) analysis to differentiate genuine Difengpi from potential adulterants, resulting in the identification of specific DNA mini-barcodes that effectively distinguished this herb from close relatives.
- Testing on commercially available Difengpi products showed high accuracy for the identified mini-barcodes, confirming their effectiveness in ensuring the authenticity and safety of herbal remedies in the market.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!