TB diagnostic insights, progress made on point of care diagnostics and bioinformatics as an additional tool for improvement.

Despite major efforts made to control tuberculosis disease (TB), this disease continues to present a major global health challenge and drug resistance is continuously growing. TB is caused by Mycobacterium tuberculosis and spreads exclusively via human-to-human contact transmission. Therefore, early detection and diagnosis for proper treatment with active TB have a great impact on public health. Regardless, most people in developing countries with TB or TB-associated symptoms do not have access to an adequate initial diagnosis. Available bacteriologic-based techniques are either inefficient or may require a longer turnaround time from the laboratory. Contemporarily, non-bacteriologic based methods have both questionable sensitivity and specificity and while others cannot distinguish between active and latent TB. Thus, additional efforts have been made to find accurate diagnostic tests for TB. Herein, we review the available methods used for TB diagnosis, and in addition, we explore point of care (POC) diagnostics as an alternative way to develop TB diagnostic tests and further evaluate whether bioinformatics can be used as an additional screening tool for identification of possible TB biomarkers for the development of POC TB diagnostics, which is part of our research focus.