NADP or CO reduction by -encoded hydrogenase through interaction with formate dehydrogenase 3 in the hyperthermophilic archaeon NA1.

The strategy using structural homology with the help of structure prediction by AlphaFold was very successful in finding potential targets for the -encoded hydrogenase of NA1. The finding that the hydrogenase can interact with FdhB to reduce the cofactor NAD(P) is significant in that the enzyme can function to supply reducing equivalents, just as F-reducing hydrogenases in methanogens use coenzyme F as an electron carrier. Additionally, it was identified that NA1 could produce formate from H and CO by the concerted action of -encoded hydrogenase and formate dehydrogenase Fdh3.