Genome Analysis of and Its Potential Progenitor Species Using Oligo-FISH.

The genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of and its relative diploid species. St-80 developed from and pDb12H developed from were used as probes in fluorescence in situ hybridization (FISH) to classify the chromosomes of into three groups, expressed as . A combined multiplex oligonucleotide probe, including pSc119.2-1, (GAA), AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten accessions of . Variability among and within the studied accessions of intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that had largely been contributed from , but not the present-day ; IWG had only one genome, , which was related to either or ; and was contributed from the genus by hybridization with or .