Genome-Wide Identification and Expression Analysis of the Copper Transporter (/) Gene Family in , a Typical Mangrove Plant.

Int J Mol Sci

Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.

Published: October 2023

AI Article Synopsis

  • The copper transporter gene family is essential for regulating copper levels in various species by transporting copper across cell membranes.
  • A genome-wide study identified four specific copper transporter genes in a certain genome, analyzing their structure, organization, and response to environmental factors.
  • The research revealed that these genes have distinct expression patterns when exposed to copper, along with specific regulatory elements related to hormones, stress, and light, setting the stage for future investigations into their roles.

Article Abstract

The copper transporter (/) gene family plays a critical part in maintaining the balance of the metal, and many diverse species depend on to move copper (Cu) across the cell membrane. In , , , , , , and a genome-wide study of the COPT protein family was performed. To understand the major roles of the gene family in (), a genome-wide study identified four genes in the genome for the first time. The domain and 3D structural variation, phylogenetic tree, chromosomal distributions, gene structure, motif analysis, subcellular localization, cis-regulatory elements, synteny and duplication analysis, and expression profiles in leaves and Cu were all investigated in this research. Structural and sequence investigations show that most have three transmembrane domains (TMDs). According to phylogenetic research, these might be divided into two subgroups, just like . gene segmental duplications and positive selection pressure were discovered by universal analysis. According to gene structure and motif analysis, most genes showed consistent exon-intron and motif organization within the same group. In addition, we found five hormones and four stress- and seven light-responsive cis-elements in the promoters. The expression studies revealed that all four genes changed their expression levels in response to copper (CuCl) treatments. In summary, our study offers a thorough overview of the gene family's expression pattern and functional diversity, making it easier to characterize each gene's function in the future.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10648262PMC
http://dx.doi.org/10.3390/ijms242115579DOI Listing

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