Gastric cancer (GC) remains a prevalent malignancy with high morbidity and mortality. CDKN1A interacting zinc finger protein 1 (CIZ1) has been demonstrated to have oncogenic functions in the development of cancers. We detected CIZ1 expression via quantitative real-time PCR (RT-qPCR). The protein level of CIZ1 was measured through Western blot. We noticed that CIZ1 expression was markedly enhanced in GC cells. Furthermore, functional experiments including colony formation assay, EdU staining assay, transwell assay, TUNEL staining assay and flow cytometry analysis uncovered that CIZ1 silencing attenuated cell malignant phenotypes in GC. Through bioinformatics tools and mechanism assays, we explored the up-stream mechanism of CIZ1 and determined that CIZ1 was modulated by FBXL19 antisense RNA 1 (FBXL19-AS1) and microRNA-339-3p (miR-339-3p). Additionally, miR-339-3p exerted a negative role on GC development in vitro, and FBXL19-AS1 depletion also had the inhibitory impacts on the progression of GC in vitro. Eventually, the finding that CIZ1 overexpression reversed the effects of FBXL19-AS1 silencing on GC development was validated by rescue assays. In a word, CIZ1 functioned as a tumor promoter in GC, indicating that CIZ1 might be a promising target for GC treatment.
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| http://dx.doi.org/10.1016/j.heliyon.2023.e21061 | DOI Listing |
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Article Synopsis
- CIZ1 is a protein that stabilizes epigenetic states, particularly in the inactive X chromosome of females, and its deletion in mice leads to hyperproliferation, indicating a link to various cancers.
- In CIZ1-null murine fibroblasts, there is reduced H4K20me1, resulting in compromised nuclear condensation when entering a resting state, affecting genes related to DNA repair despite overall transcriptional repression being maintained.
- The study highlights the critical role of CIZ1 in maintaining chromatin structure during quiescence cycles, with its absence leading to genome instability, making cells more vulnerable to damage and potential transformation into cancerous colonies.
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