AI Article Synopsis
- Infection by Bombyx mori nucleopolyhedrovirus (BmNPV) leads to significant economic losses in silkworm farming, necessitating a quick and efficient detection method.
- The study developed a recombinase-aided amplification (RAA) technique that can amplify BmNPV DNA in just 30 minutes at 37°C, which is then coupled with a lateral flow dipstick (LFD) for rapid results.
- The RAA-LFD method offers high sensitivity, specifically detects BmNPV without interfering with other pathogens, and is more cost-effective and suitable for on-site detection compared to traditional PCR methods.
Article Abstract
The infection of Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the main causes of economic losses in sericulture. Thus, it is essential to establish rapid and effective method for BmNPV detection. In the present study, we have developed a recombinase-aided amplification (RAA) to amplify the BmNPV genomic DNA at 37 °C within 30 min, and achieved a rapid detection method by coupling with a lateral flow dipstick (LFD). The RAA-LFD method had a satisfactory detection limit of 6 copies/μL of recombinant plasmid pMD19-T-IE1, and BmNPV infection of silkworm can be detected 12 h post-infection. This method was highly specific for BmNPV, and without cross-reactivity to other silkworm pathogens. In contrast to conventional polymerase chain reaction (PCR), the RAA-LFD assay showed higher sensitivity, cost-saving, and especially is apt to on-site detection of BmNPV infection in the sericulture production.
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| http://dx.doi.org/10.1007/s12223-023-01102-7 | DOI Listing |
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