The ameliorative effects of Sida acuta leaf meal (SALM) and vitamin C on the serum pro-inflammatory and anti-inflammatory cytokines as well as DNA damage of cocks fed aflatoxin B (AFB) contaminated diets were examined. The experiment was a completely randomized design with a total of 250 sexually mature Isa White cocks aged 24 weeks, randomly allotted into five experimental diets; each diet contained 5 replicates with 10 roosters. The diets were A (control/basal diet), B (A + 1 mg/kg AFB), C (B + 200 mg/kg vitamin C), D (B + 2.5 g/kg SALM) and E (B + 5.0 g/kg SALM). Fresh and clean water was also provided for the whole experimental period of twelve weeks. Inclusion of 1 mg/kg AFB without vitamin C or SALM increased TNF-α and IL-1β as well as 8-OHdG and NF-κB in the serum significantly (P < 0.05) among the cocks on diet B. However, the fortification of AFB contaminated diets with vitamin C and SALM depressed serum TNF-α, IL-1β, 8-OHdG and NF-κB concentrations of the cocks significantly (P < 0.05). Conversely, serum IL-4 and IL-10 in birds given 1 mg/kg AFB without vitamin C or SALM decreased significantly (P < 0.05) in comparison with the roosters on the control. However, improvements (P < 0.05) in IL-4 and IL-10 concentrations with corresponding reduction (P < 0.05) in TNF-α, IL-1β, 8-OHdG and NF-κB concentrations were recorded among cocks fed Diets C, D and E, respectively. Therefore, dietary addition of SALM at the level used in this study was beneficial and has comparable effects with inorganic antioxidant (C vitamin) by significantly reducing the inflammatory cytokines and oxidative damage biomarkers as well as enhancing the anti-inflammatory cytokines thereby promoting the health status of the cocks fed AFB contaminated ration.

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http://dx.doi.org/10.1016/j.toxicon.2023.107330DOI Listing

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