The kinetic hydrate inhibitor (KHI) was developed to prevent the formation of undesirable gas hydrate crystals in natural gas pipelines. Studies of antifreeze proteins (AFPs) are gaining attention in the natural gas research field due to their performance in crystal growth inhibition, excellent biodegradation, and low toxicity. Studies of AFPs may provide clues for developing future commercial KHIs used offshore. This chapter presents a simple method of evaluating AFP inhibitory performance as a KHI on tetrahydrofuran (THF) hydrate growth with a unidirectional growth apparatus.
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