Many ice-binding proteins can stimulate nucleation of ice from supercooled liquid water. Quantification of ice nucleation usually involves cooling a population of droplets and observing their freezing temperatures. Here, a method for measuring the freezing temperatures of microliter-scale droplets is described and its application to quantifying a substances' ice nucleation activity discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-3503-2_9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Impact of Siberian Wildfires on Ice-Nucleating Particle Concentrations over the Northwestern Pacific.
Environ Sci Technol
January 2025
Research Institute for Global Change, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokohama, Kanagawa 236-0001, Japan.
Ice-nucleating particles (INPs) significantly influence aerosol-cloud precipitation interactions at regional and global scales. However, information regarding the concentrations and origins of INPs over the open ocean, particularly at high latitudes, remains insufficient due to access difficulties. In this study, we investigated the concentrations and origins of INPs over the western North Pacific to the Arctic Ocean through ship-borne observations conducted in the early autumn of 2016.
View Article and Find Full Text PDFBioaerosol Characterization with Vibrational Spectroscopy: Overcoming Fluorescence with Photothermal Infrared (PTIR) Spectroscopy.
J Phys Chem A
January 2025
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
Aerosols containing biological material (i.e., bioaerosols) impact public health by transporting toxins, allergens, and diseases and impact the climate by nucleating ice crystals and cloud droplets.
View Article and Find Full Text PDFMechanism of Water Freezing in Solutions: Solutes Affect the Formation of Critical Ice Nuclei.
Nano Lett
January 2025
"The Belt and Road Initiative" Advanced Materials International Joint Research Center of Hebei Province, School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300401, China.
The microscopic mechanisms by which solutes modulate water freezing are fundamental for controlling the freezing of various environmental and cryobiotic systems. Although our understanding of the initiation mechanisms of pure water freezing is becoming clearer, the microscopic pictures regarding ice nucleation in complex systems such as solutions still rely on theory assumption and empirical formulation. Here, we experimentally demonstrate that solutes modulate water freezing through affecting critical ice nucleus formation.
View Article and Find Full Text PDFFully Atomistic Molecular Dynamics Simulation of Ice Nucleation Near an Antifreeze Protein.
J Am Chem Soc
January 2025
Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong, Kowloon 999077, China.
Heterogeneous ice nucleation is a widespread phenomenon in nature. Despite extensive research on ice nucleation near biological antifreeze proteins, a probe for ice nucleation and growth processes at the atomic level is still lacking. Herein, we present simulation evidence of the heterogeneous ice nucleation process on the ice-binding surface (IBS) of the antifreeze protein (TmAFP).
View Article and Find Full Text PDFOptimization of canine sperm cryopreservation by focusing on glycerol concentration and freezing rate.
Vet Res Commun
January 2025
Faculty of Agriculture, University Farm, Utsunomiya University, Tochigi, 321-4415, Japan.
The purpose of this study was to improve the quality of frozen-thawed canine spermatozoa through the optimization of glycerol concentration (GC) and freezing rate in the semen freezing protocol. Ejaculates from nine dogs were diluted with an extender containing 0%, 1.5%, 3%, 6%, or 9% glycerol.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!