Detection of environmental nanoplastics via surface-enhanced Raman spectroscopy using high-density, ring-shaped nanogap arrays.

Micro- and nano-plastics (MNPs) are global contaminants of growing concern to the ecosystem and human health. In-the-field detection and identification of environmental micro- and nano-plastics (e-MNPs) is critical for monitoring the spread and effects of e-MNPs but is challenging due to the dearth of suitable analytical techniques, especially in the sub-micron size range. Here we show that thin gold films patterned with a dense, hexagonal array of ring-shaped nanogaps (RSNs) can be used as active substrates for the sensitive detection of micro- and nano-plastics by surface-enhanced Raman spectroscopy (SERS), requiring only small sample volumes and no significant sample preparation. By drop-casting 0.2-μL aqueous test samples onto the SERS substrates, 50-nm polystyrene (PS) nanoparticles could be determined via Raman spectroscopy at concentrations down to 1 μg/mL. The substrates were successfully applied to the detection and identification of ∼100-nm polypropylene e-MNPs in filtered drinking water and ∼100-nm polyethylene terephthalate (PET) e-MNPs in filtered wash-water from a freshly cleaned PET-based infant feeding bottle.