Background: Platelet-rich fibrin (PRF) is used to prepare "sticky bone" by combining it with bone graft material. The present study investigated the ability of different bone grafts to absorb growth factors from the PRF and release them over time.

Materials And Methods: Human blood was collected from 10 healthy volunteers for liquid PRF preparation. Bovine bone, allograft (mineralized and demineralized), and synthetic bone were each mixed with the PRF to prepare a sticky bone. All sticky bone samples were incubated for up to 4 days. The absorption and release pattern kinetics of two selective growth factors within the PRF-platelet-derived growth factor (PDGF) and bone morphogenetic protein-2 (BMP-2)-were quantified with immunofluorescence staining and enzyme-linked immunoassay (ELISA) testing.

Results: All bone graft materials adsorbed the examined growth factors from the PRF. β-TCP showed the highest adsorption levels, followed by the xenograft, and the allografts showed the lowest adsorption levels. Furthermore, PDGF showed a fast-release pattern from the grafts, whereas BMP-2 was released at a later stage. Similar to the adsorption pattern, the β-TCP and xenograft were better able to sustain the release of the PRF growth factors from the graft than the allografts.

Conclusions: The adsorption of PDGF and BMP-2 differ between graft materials, with superior results for β-TCP, followed by xenograft, then allograft materials.

Download full-text PDF

Source
http://dx.doi.org/10.11607/jomi.10529DOI Listing

Publication Analysis

Top Keywords

growth factors
20
bone
9
release pattern
8
bone grafts
8
platelet-rich fibrin
8
prf prepare
8
bone graft
8
factors prf
8
sticky bone
8
graft materials
8

Similar Publications

Autism spectrum disorder (ASD) is a prevalent neurodevelopmental condition affecting a substantial number of children globally, characterized by diverse aetiologies, including genetic and environmental factors. Emerging research suggests that neurovascular dysregulation during development could significantly contribute to autism. This review synthesizes the potential role of vascular abnormalities in the pathogenesis of ASD and explores insights from studies on valproic acid (VPA) exposure during neural tube development.

View Article and Find Full Text PDF

Importance: Cardiovascular disease (CVD) and cancer are the leading causes of mortality in the US. Large-scale population-based and mechanistic studies support a direct effect of CVD on accelerated tumor growth and spread, specifically in breast cancer.

Objective: To assess whether individuals presenting with advanced breast cancers are more likely to have prevalent CVD compared with those with early-stage breast cancers at the time of diagnosis.

View Article and Find Full Text PDF

Purpose: Inflammatory processes have been involved in diabetic retinopathy (DR). Interleukin (IL)-17A, a pro-inflammatory cytokine, is associated with DR occurrence and development. However, mechanisms underlying the IL-17A impact on DR need further investigations.

View Article and Find Full Text PDF

is a foodborne pathogen linked to severe infections in infants and often associated with contaminated powdered infant formula. The RecA protein, a key player in DNA repair and recombination, also influences bacterial resilience and virulence. This study investigated the impact of deletion on the pathogenicity and environmental stress tolerance of BAA-894.

View Article and Find Full Text PDF

Unlabelled: RamA is an intrinsic regulator in , belonging to the AraC family of transcription factors and conferring a multidrug resistance phenotype, especially for tetracycline-class antibiotics. The ATP-binding cassette transporters MlaFEDCB in bacteria play essential roles in functions essential for cell survival and intrinsic resistance to many antibiotics. We found deletion of resulted in a fivefold decrease in the transcriptional levels of the operon.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!