Effect of silencing the E74B gene on the development and metamorphosis of Helicoverpa armigera.

Background: The growth and development transition of insects are mainly mediated by ecdysone. As one of the ecdysone-induced transcription factors, E74 is involved in many physiological processes of insect growth and development. However, E74 and its function in Helicoverpa armigera remains unclear.

Results: In this study, E74B, a subtype of the E74, was identified for the first time in H. armigera. Bioinformatics analysis showed that H. armigera E74B shared the highest homology with E74B in Bombyx mori, which belongs to the E26 transformation-specific (ETS) superfamily. The expression profile showed that the transcription level of HaE74B increased in the late stages of fourth to sixth instars compared with the early stages; it was also high in the pupa and midgut. Moreover, we investigated the function of HaE74B through RNA interference and 20E rescue experiments. The results showed silencing of E74B affected the molting and growth of larvae, resulting in the death of more than 60% of larvae. In addition, it also seriously affected the metamorphosis of H. armigera, which reduced the pupae rate, the eclosion rate of the pupae, and fecundity. Application of 20E partially restored the defects in the molting, development and pupae rate of H. armigera.

Conclusion: Taken together, these results demonstrated that HaE74B plays a critical role in the growth, development, and metamorphosis of H. armigera, which serves as a molecular target and sets out a theoretical foundation for RNAi-mediated control of this key pest. © 2023 Society of Chemical Industry.