The understanding and manipulation of microbial communities toward the conversion of lignocellulose and plastics are topics of interest in microbial ecology and biotechnology. In this study, the polymer-degrading capability of a minimal lignocellulolytic microbial consortium (MELMC) was explored by genome-resolved metagenomics. The MELMC was mostly composed (>90%) of three bacterial members (Pseudomonas protegens; Pristimantibacillus lignocellulolyticus gen. nov., sp. nov; and Ochrobactrum gambitense sp. nov) recognized by their high-quality metagenome-assembled genomes (MAGs). Functional annotation of these MAGs revealed that Pr. lignocellulolyticus could be involved in cellulose and xylan deconstruction, whereas Ps. protegens could catabolize lignin-derived chemical compounds. The capacity of the MELMC to transform synthetic plastics was assessed by two strategies: (i) annotation of MAGs against databases containing plastic-transforming enzymes; and (ii) predicting enzymatic activity based on chemical structural similarities between lignin- and plastics-derived chemical compounds, using Simplified Molecular-Input Line-Entry System and Tanimoto coefficients. Enzymes involved in the depolymerization of polyurethane and polybutylene adipate terephthalate were found to be encoded by Ps. protegens, which could catabolize phthalates and terephthalic acid. The axenic culture of Ps. protegens grew on polyhydroxyalkanoate (PHA) nanoparticles and might be a suitable species for the industrial production of PHAs in the context of lignin and plastic upcycling.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723784 | PMC |
| http://dx.doi.org/10.1038/s43705-022-00176-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enteric methane research and mitigation strategies for pastoral-based beef cattle production systems.
Front Vet Sci
December 2022
Teagasc, Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Dunsany, Ireland.
Ruminant livestock play a key role in global society through the conversion of lignocellulolytic plant matter into high-quality sources of protein for human consumption. However, as a consequence of the digestive physiology of ruminant species, methane (CH), which originates as a byproduct of enteric fermentation, is accountable for 40% of global agriculture's carbon footprint and ~6% of global greenhouse gas (GHG) emissions. Therefore, meeting the increasing demand for animal protein associated with a growing global population while reducing the GHG intensity of ruminant production will be a challenge for both the livestock industry and the research community.
View Article and Find Full Text PDFNovel bacterial taxa in a minimal lignocellulolytic consortium and their potential for lignin and plastics transformation.
ISME Commun
September 2022
Microbiomes and Bioenergy Research Group, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
The understanding and manipulation of microbial communities toward the conversion of lignocellulose and plastics are topics of interest in microbial ecology and biotechnology. In this study, the polymer-degrading capability of a minimal lignocellulolytic microbial consortium (MELMC) was explored by genome-resolved metagenomics. The MELMC was mostly composed (>90%) of three bacterial members (Pseudomonas protegens; Pristimantibacillus lignocellulolyticus gen.
View Article and Find Full Text PDFApplication of Phytochemicals To Combat Fungal Pathogens of Pulses: An Approach toward Inhibition of Fungal Propagation and Invasin Activity.
J Agric Food Chem
June 2022
Department of Microbiology, Vidyasagar University, Midnapore, West Bengal 721102, India.
The present study represented an innovative strategy for inactivating the secreted invasins (lignocellulolytic enzymes) of fungal phytopathogens using natural phytochemicals to combat fungal infection to the pulses. A fungal pathogen ( SKP1) was isolated from the white lentil (), which has the ability to synthesize different lignocellulolytic enzymes. An in silico docking study elucidated that quercetin, naringin, epigallocatechin gallate, curcumin, and cinnamic acid were the prime efficient phytochemicals to inhibit the activity of fungal invasive enzymes like endoglucanase, -1,4-β-xylanase, and glucoamylase.
View Article and Find Full Text PDFFungal lignocellulolytic enzymes and lignocellulose: A critical review on their contribution to multiproduct biorefinery and global biofuel research.
Int J Biol Macromol
December 2021
Laboratory of Enzymology and Recombinant DNA Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India. Electronic address:
The continuous increase in the global energy demand has diminished fossil fuel reserves and elevated the risk of environmental deterioration and human health. Biorefinery processes involved in producing bio-based energy-enriched chemicals have paved way to meet the energy demands. Compared to the thermochemical processes, fungal system biorefinery processes seems to be a promising approach for lignocellulose conversion.
View Article and Find Full Text PDFCross-feeding and wheat straw extractives enhance growth of Clostridium thermocellum-containing co-cultures for consolidated bioprocessing.
Bioprocess Biosyst Eng
April 2021
Department of Microbiology, University of Manitoba, 213 Buller Building, Winnipeg, MB, R3T 2N2, Canada.
Co-cultures consisting of three thermophilic and lignocellulolytic bacteria, namely Clostridium thermocellum, C. stercorarium, and Thermoanaerobacter thermohydrosulfuricus, degrade lignocellulosic material in a synergistic manner. When cultured in a defined minimal medium two of the members appeared to be auxotrophic and unable to grow, but the growth of all species was observed in all co-culture combinations, indicating cross-feeding of unidentified growth factors between the members.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!