AI Article Synopsis
- Mass spectrometry-based metaproteomics is a new research area for understanding microbial functionality, overcoming limitations of previous methods.
- A novel untargeted DIA metaproteomics tool has been developed that does not require additional DDA data, simplifying the analysis process.
- The tool, called glaDIAtor, is available as open-source software with a user-friendly web-based interface to encourage broad usage.
Article Abstract
Mass spectrometry-based metaproteomics is a relatively new field of research that enables the characterization of the functionality of microbiota. Recently, we demonstrated the applicability of data-independent acquisition (DIA) mass spectrometry to the analysis of complex metaproteomic samples. This allowed us to circumvent many of the drawbacks of the previously used data-dependent acquisition (DDA) mass spectrometry, mainly the limited reproducibility when analyzing samples with complex microbial composition. However, the DDA-assisted DIA approach still required additional DDA data on the samples to assist the analysis. Here, we introduce, for the first time, an untargeted DIA metaproteomics tool that does not require any DDA data, but instead generates a pseudospectral library directly from the DIA data. This reduces the amount of required mass spectrometry data to a single DIA run per sample. The new DIA-only metaproteomics approach is implemented as a new open-source software package named glaDIAtor, including a modern web-based graphical user interface to facilitate wide use of the tool by the community.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723653 | PMC |
| http://dx.doi.org/10.1038/s43705-022-00137-0 | DOI Listing |
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