AI Article Synopsis

  • The study investigates differences in vitality between in vitro produced (IVP) and ex vivo derived bovine embryos, highlighting that IVP embryos tend to be less viable.
  • Research focuses on how mitochondrial function relates to embryo development, which has not been extensively studied before.
  • Findings reveal that embryo quality is influenced by environmental conditions and developmental timing, with higher-quality embryos showing distinct respiration patterns and optimal mitochondrial activity.

Article Abstract

The major limitation of the widespread use of IVP derived embryos is their consistent deficiencies in vitality when compared with their ex vivo derived counterparts. Although embryo metabolism is considered a useful metric of embryo quality, research connecting mitochondrial function with the developmental capacity of embryos is still lacking. Therefore, the aim of the present study was to analyse bovine embryo respiration signatures in relation to developmental capacity. This was achieved by taking advantage of two generally accepted metrics for developmental capacity: (I) environmental conditions during development (vivo vs. vitro) and (II) developmental kinetics (day 7 vs. day 8 blastocysts). Our study showed that the developmental environment affected total embryo oxygen consumption while different morphokinetics illustrating the embryo qualities correlate with maximal mitochondrial respiration, mitochondrial spare capacity, ATP-linked respiration as well as efficiency of ATP generation. This respiration fingerprint for high embryo quality is reflected by relatively lower lipid contents and relatively higher ROS contents. In summary, the results of the present study extend the existing knowledge on the relationship between bovine embryo quality and the signature of mitochondrial respiration by considering contrasting developmental environments as well as different embryo morphokinetics.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10632430PMC
http://dx.doi.org/10.1038/s41598-023-45691-2DOI Listing

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