Solvent property of air-water interface was evaluated based on the fluorescence spectra of 1,2'-dinaphthylamine in water containing ultrafine bubbles (average diameter: 103 nm, standard deviation: 38 nm). Among naphthylamine derivatives whose fluorescence spectra were responsive to microscopic hydrophobicity, 1,2'-dinaphthylamine (DN) was selected because its wavelength of the maximum emission (λ) was significantly dependent on the concentration and microenvironment of the ultrafine bubble. The λ value of DN in water was 486 nm, while it shifted to shorter wavelength (408 nm) in the presence of 1.09 × 10 mL of ultrafine bubbles. The shift of λ value indicates that DN adsorbs on the surfaces of ultrafine bubbles and exists in hydrophobic region rather than in bulk water. By comparing with the λ values in different solvents, the surface of ultrafine bubble was found to have similar solvent property to ethyl ether or ethyl acetate that are widely used as extracting solvents for hydrophobic organic compounds.
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