Objective: Improvement of methods for studying the processes of demineralization of hard tissues of temporary teeth.
Material And Methods: The study included primaries second molars (=11). Samples of primary teeth were placed in a test tube with a demineralizing solution for - 1, 4, 8, 21 and 31 days. The of primary teeth samples were examined using methods - laser induced fluorescence (LIF) and autofluorescence microscopy (AFM). Assessment of the degree of demineralization of samples of temporary teeth was carried out according to the score scale developed by us.
Results: The enamel of the samples is demineralized slowly and evenly for up to 8 days with minimal objective signs, starting from the 8th day of the experiment, there is a significant increase in demineralization indicators. By the 21st day, the peak of demineralization is reached with partial dissolution of the enamel, an increase in the fluorescence effect to 80 UE, and reaches a maximum of 4 points on the evaluation scale. Dentin's hard tissues are demineralized gradually without "sudden jumps" in the fluorescence effect and at the same rate throughout the experiment, reaching a maximum on 31 days (30 UE - LIF). Dentin demineralization is characterized by less dissolution, however, the phenomenon of delamination is determined by the type of exfoliation of the organic dentin matrix, starting from the 21st day of the experiment.
Conclusion: Enamel and dentin of deciduous teeth demineralize at different rates and have a characteristic specificity of morphological changes. Logistic regression analysis showed the consistency of the classifier for the predictive accuracy of each unit of the proposed scale for assessing the degree of demineralization of temporary teeth samples.
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http://dx.doi.org/10.17116/stomat20231020516 | DOI Listing |
Molecules
December 2024
Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
Mild hypophosphatasia (HPP) can be difficult to distinguish from other bone disorders in the absence of typical symptoms such as the premature loss of primary teeth. Therefore, this study aimed to analyze the crystallinity of hydroxyapatite (HAp) and the three-dimensional structure of collagen in HPP teeth at the molecular level and to search for new biomarkers of HPP. Raman spectroscopy was used to investigate the molecular structure, composition, and mechanical properties of primary teeth from healthy individuals and patients with HPP.
View Article and Find Full Text PDFLife (Basel)
December 2024
Department of Microbiology, Faculty of Dentistry, Nuh Naci Yazgan University, Kayseri 38170, Turkey.
Background: Effective management of primary apical periodontitis depends on understanding the dynamic interactions within the root canal microbiome. This study aimed to investigate the effect of sequential antimicrobial phases on the root canal microbiome during a two-visit treatment approach, with a focus on calcium hydroxide medication.
Methods: Samples were collected from three teeth across four treatment phases: initial infection (S1), after chemomechanical preparation (S2), after intracanal medication (S3), and after a final flush (S4).
Children (Basel)
December 2024
Department of Preventive Dental Sciences, College of Dentistry, Prince Sattam bin Abdulaziz University, Alkharj 11942, Saudi Arabia.
Background: This study aimed to assess the effectiveness of laser photobiomodulation (PBM) in reducing postoperative pain following single-visit endodontic treatment in children aged 5-9 years.
Methods: Forty children aged 5-9 years with acute irreversible pulpitis in deciduous molars requiring single-visit pulpectomy were included in the study. Pulpectomy was performed according to a standard endodontic protocol.
Children (Basel)
November 2024
Department of Pediatric Dentistry, Faculty of Dental Medicine, Hebrew University, Hadassah Medical Center, P.O. Box 12272, Jerusalem 91120, Israel.
Objectives: The present prospective study aimed to compare near-infrared light reflection (NIRI) and bitewing radiographs (BWR) images to detect proximal caries in primary teeth.
Methods: 71 children underwent routine BWR, and scans were performed using an intra-oral scanner (iTero Element 5D, Align Technology, Tempe, AZ, USA), including a near-infrared light source (850 nm) and sensor. Five specialist pediatric dentists examined the NIRI and BWR images.
Biomedicines
December 2024
Department of Orthodontics, University of Münster, 48149 Münster, Germany.
There is a lack of evidence for the relationship between sweat pores and tooth agenesis. The aim of this study was to compare sweat pore density on fingertips between a group of patients with oligodontia and a control group without tooth agenesis. This parallel-group controlled clinical trial included 28 patients.
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