Bioprotection of Olive Trees Against Verticillium Wilt by Pomegranate and Carob Extracts.

Bioprotection through the use of plant extracts is an environmentally friendly strategy in crop protection. Effective control of Verticillium wilt of olive (; VWO), caused by , has proven challenging because of the ineffectiveness of chemicals, which makes it necessary to search for new control tools. Thus, the aim of this study was to evaluate the effect of pomegranate () and carob () extracts on VWO. Extracts derived from pomegranate peels and carob pods and leaves were obtained using ethanol, methanol, or ethyl acetate as a solvent. A targeted analysis of their metabolite composition was performed using QTRAP ultrahigh-performance liquid chromatography with mass spectrometry. Remarkably, gallic acid was detected in all extracts at a high concentration. The effect of the extracts on the mycelial growth and on the germination of conidia and microsclerotia of was evaluated by in vitro sensitivity tests at various doses: 0 (control), 3, 30, 300, and 3,000 mg of extract/liter. Extracts obtained with ethanol or methanol significantly reduced the viability of structures when applied at the highest dose, while those obtained with ethyl acetate were ineffective across all doses. The most effective extracts, as determined in vitro, were then evaluated against the disease in olive plants. Potted plants of the cultivar Picual were treated by spraying (foliar application) or irrigation (root application) of extracts at 3,000 mg/liter, followed by inoculation with . The results indicated that foliar applications were ineffective, while root treatments with pomegranate peel or carob leaf extracts were more effective in reducing disease severity, regardless of the solvent, compared with that of the untreated control.