Bacterial secretion systems allow the transport of proteins, called effectors, as well as external machine components in the extracellular medium or directly into target cells. Comparison of the secretome, i.e., the proteins released in the culture medium, of wild-type and mutant cells provides information on the secretion profile. In addition, mass spectrometry analyses of the culture supernatant of bacteria grown in liquid culture under secreting conditions allow the identification of secretion systems substrates. Upon identification of the substrates, the secretion profile serves as a tool to test the functionality of secretion systems. Here, we present a classical method used to concentrate the culture supernatant, based on TCA precipitation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-3445-5_32 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Maize drought protection by Azospirillum argentinense Az19 requires bacterial trehalose accumulation.
Appl Microbiol Biotechnol
December 2024
Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP), Ruta Provincial 226 Km 73.5, B7620, Balcarce, Buenos Aires, Argentina.
Azospirillum argentinense Az19 is an osmotolerant plant growth-promoting bacterium that protects maize plants from drought. In this work, we explored the role of trehalose in the superior performance of Az19 under stress. The trehalase-coding gene treF was constitutively expressed in Az19 through a miniTn7 system.
View Article and Find Full Text PDFPassive accumulation of alkaloids in inconspicuously colored frogs refines the evolutionary paradigm of acquired chemical defenses.
Elife
December 2024
Department of Chemistry and Physics, Indiana State University, Terre Haute, United States.
Understanding the origins of novel, complex phenotypes is a major goal in evolutionary biology. Poison frogs of the family Dendrobatidae have evolved the novel ability to acquire alkaloids from their diet for chemical defense at least three times. However, taxon sampling for alkaloids has been biased towards colorful species, without similar attention paid to inconspicuous ones that are often assumed to be undefended.
View Article and Find Full Text PDFTarget protein identification in live cells and organisms with a non-diffusive proximity tagging system.
Elife
December 2024
Department of Neurology, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Identifying target proteins for bioactive molecules is essential for understanding their mechanisms, developing improved derivatives, and minimizing off-target effects. Despite advances in target identification (target-ID) technologies, significant challenges remain, impeding drug development. Most target-ID methods use cell lysates, but maintaining an intact cellular context is vital for capturing specific drug-protein interactions, such as those with transient protein complexes and membrane-associated proteins.
View Article and Find Full Text PDFErgogenic and Sympathomimetic Effects of Yohimbine: A Review.
Neurol Int
December 2024
Department of Human Studies, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
The purpose of this review is to compile and discuss available evidence in humans on the efficacy of YHM supplementation on performance in different exercise modalities. Yohimbine (YHM) is a naturally occurring alkaloid that induces increases in sympathetic nervous system (SNS) activation effectively initiating "fight or flight" responses. In supplement form, YHM is commonly sold as an isolated product or combined into multi-ingredient exercise supplements and is widely consumed in fitness settings despite the lack of empirical support until recently.
View Article and Find Full Text PDFIntegrated Profiling of Extracellular Vesicle microRNA Impact on Trabecular Meshwork mRNA Expression: Insights From Microarray Analysis.
Invest Ophthalmol Vis Sci
December 2024
Department of Software and Information Systems Engineering, Faculty of Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Purpose: Extracellular vesicles (EVs) secreted by non-pigmented ciliary epithelial (NPCE) cells under oxidative stress may contribute to primary open-angle glaucoma (POAG) pathogenesis by altering gene expression in human trabecular meshwork (HTM) cells. This study investigated the impact of microRNAs (miRNAs) carried by NPCE-derived EVs on HTM cell gene expression under oxidative stress conditions.
Methods: NPCE cells were exposed to oxidative stress, and EVs were isolated from control and stressed cells.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!