AI Article Synopsis
- The rising issue of drug resistance and new pathogens poses a significant global health challenge, highlighting the need for better tools to study fungal drug resistance.
- Previous research mainly relied on limited methods for genetic modification in certain strains, which restricted understanding of fungal resistance mechanisms.
- This study introduced a CRISPR-based system that effectively modifies genes linked to drug resistance, enhancing our knowledge of specific genes related to azole resistance and broadening the options for investigating complex pathways in fungal pathogens.
Article Abstract
The increasing problem of drug resistance and emerging pathogens is an urgent global health problem that necessitates the development and expansion of tools for studying fungal drug resistance and pathogenesis. Prior studies in , , and have been mainly limited to the use of and for genetic manipulation in prototrophic strains and clinical isolates. In this study, we demonstrated that and/or drug resistance cassettes when coupled with a CRISPR-ribonucleoprotein (RNP)-based system can be efficiently utilized for deleting or modifying genes in the ergosterol pathway of , , and . Moreover, the utility of these tools has provided new insights into genes and their relationship to azole resistance in . Overall, we have expanded the toolkit for pathogens to increase the versatility of genetically modifying complex pathways involved in drug resistance and pathogenesis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10732037 | PMC |
| http://dx.doi.org/10.1128/msphere.00311-23 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!